胃癌的细胞增殖、DNA增殖分数和倍性与患者生存的关系  

Relationship between proliferation, DNA proliferative fraction, DNA ploidy and survival in gastric cancer patients

作　　者：陈金明[1] 李伟华[2] 周海波[1] 

机构地区：[1]浙江大学医学院附属第二医院外科,浙江杭州310009 [2]福建省立医院外科,福建福州350000

出　　处：《中国病理生理杂志》2005年第8期1562-1565,共4页Chinese Journal of Pathophysiology

摘　　要：目的:探讨胃癌细胞增殖、DNA增殖分数(S+G2/M期)和倍性与胃癌患者生存和生物学特性的关系。方法:应用流式细胞术,分别分析胃癌细胞群体的增殖、DNA增殖分数和倍性。结果:胃癌细胞群体的增殖和增殖分数之间无明显相关关系(P>0·05);它们与胃癌患者的生存亦无明显相关关系(P>0·05)。胃癌细胞群体的增殖和DNA倍性与胃癌临床病理参数之间无明显相关关系(P>0·05)。胃癌患者DNA二倍体组和异倍体组Kaplan-Meier生存曲线经Log-rank检验有统计学差异(P<0·05)。结论:胃癌细胞的增殖和增殖分数与胃癌患者的生存无明显关系;DNA倍性分析对胃癌预后的判断具有重要参考价值。AIM： To evaluate the relationship between cell proliferation, DNA proliferative fraction （S ＋ G2/M）, DNA ploidy, biological features and survival in gastric cancer patients. METHODS： Proliferation, DNA proliferative fraction and DNA ploidy were analyzed by flow cytometry using standard techniques. RESULTS： No correlation between proliferation and DNA proliferative fraction （ P 〉 0.05） was observed. Likewise, there was no correlation between proliferation, DNA proliferative fraction and survival （ P 〉 0.05）, nor was there a difference between proliferation, DNA ploidy and clinical pathological variables （ P 〉 0.05）.Using Kaplan- Meier survival curves and the Log- rank test, statistic difference was found in patients with DNA diploid tuners and those with DNA aneuploid tumors （P 〈 0.05）. CONCLUSION： Proliferation and DNA proliferative fraction in gastric cancer do not appear to correlate with survival, buf DNA ploidy can be used as a prognostic factor in gastric cancer.

关 键 词：胃肿瘤 细胞增殖 DNA增殖分数 DNA倍性 幸存 流式细胞术 

分 类 号：R73[医药卫生—肿瘤]