As_2O_3对胰腺癌细胞株的抑制作用及其机制的初步探讨  

Inhibitory effect of arsenous oxide on pancreatic cancer cell line

作　　者：史立军[1] 闫彬彬[2] 李双星[1] 孟艳玲[1] 马珊珊[3] 刘铁夫[1] 李呼伦[3] 

机构地区：[1]哈尔滨医科大学附属第一医院消化内科,黑龙江哈尔滨150086 [2]哈尔滨医科大学生理学教研室,黑龙江哈尔滨150086 [3]哈尔滨医科大学神经生物学教研室,黑龙江哈尔滨150086

出　　处：《中国病理生理杂志》2005年第8期1572-1574,共3页Chinese Journal of Pathophysiology

基　　金：黑龙江省十五攻关课题基金资助项目(No.GB02C141-02)

摘　　要：目的:探讨三氧化二砷(arsenous oxide,As2O3)对胰腺癌PC2细胞的抑制作用及其作用机制。方法:用胰腺癌PC2细胞株进行培养传代,观察As2O3作用于细胞的不同时间,对细胞生长抑制情况;利用TUNEL染色检测胰腺癌细胞凋亡率;RT-PCR方法检测survivin基因的表达。结果:①随着药物对胰腺癌细胞作用时间的延长,细胞生存率明显降低,药物作用存在时间依赖性(P<0·05,P<0·01)。②TUNEL染色证实,As2O3对胰腺癌细胞的抑制作用是以促进细胞凋亡为主。③凋亡抑制因子survivin基因在胰腺癌细胞中表达;胰腺癌细胞经As2O3作用后,抑制sur-vivin基因的表达(P<0·05)。结论:As2O3可能通过抑制凋亡抑制因子survivin基因的表达,促进细胞凋亡,从而发挥抗肿瘤的作用。AIM： To study the effect and mechanism of arsenous oxide （As2O3） on pancreatic cancer PC2 strain.METHODS： The pancreatic cancer PC2 strain was chosen in this experiment. Apoptosis was detected by TUNEL test. Expressionof survivin gene was detected by reverse transcriptase PCR. RESULTS： ① After administration of As2O3, the survival number of pancreatic cancer cells decreased significantly in a time- dependent manner （ P 〈 0.05, P 〈 0.01）. ② The fact that apoptosis was a major way of pancreatic cancer cells death after drug treatment was confirmed by TUNEL test. ③ The survivin gene was a kind of apoptosis inhibitory factor. The inhibitory rate of survivin gene expression induced by As2O3 was higher than that in control group （ P〈 0.05）. CONCLUSION： As2O3 may inhibit cancer by accelerating apoptosis through inhibition of the expression of survivin.

关 键 词：三氧化二砷 细胞凋亡 癌基因 胰腺肿瘤 

分 类 号：R363[医药卫生—病理学]