善得定抑制体外肺腺癌Spc-a1细胞生长的实验研究  

An Experimental Study on Inhibition of Sandostatin on the Growth of Lung Adenocarcinoma Cell Line Spc-a1

作　　者：徐振武[1] 苏颖[1] 黄诚[1] 林华妹[1] 艾响铃 邹长炎[1] 

机构地区：[1]福建省肿瘤医院,福建福州350014 [2]宜春市卫生学校,江西宜春336000

出　　处：《中国肿瘤》2005年第8期553-555,共3页China Cancer

基　　金：福建省卫生厅青年科学基金资助

摘　　要：[目的]研究生长抑素类似物善得定在体外对肺腺癌细胞株Spc-a1生长抑制和肺腺癌细胞凋亡的作用。[方法]以不同浓度穴0.01,0.1,1.0,10,20μg/ml雪善得定作用于肺腺癌细胞株Spc-a172h后,采用MTT法观察其抑瘤效应,采用TUNEL原位末端标记法检测肺腺癌细胞Spc-a1凋亡情况。[结果]善得定对肺腺癌Spc-a1细胞作用与药物剂量呈明显正相关(P=0.0001);10μg/ml,20μg/ml善得定对肺腺癌细胞Spc-a1抑制率分别为6.264%、7.78%。善得定0.01μg/ml,0.1μg/ml,1.0μg/ml浓度时引起肺腺癌细胞株Spc-a1凋亡发生率分别为1.89%,3.56%,4.44%。[结论]在体外善得定可抑制肺癌细胞Spc-a1生长。[Purpose] To investigate the inhibition of somatostatin analogue sandostatin on the growth and induced apoptosis in lung adenocareinoma cell line Spc-a1 .[Methods] The inhibition was examined by MTT assay and the apoptosis of Spe-a1 cells was detected by TUNEL in situ end labeling（isel） method with sandostatin at concentration of 0.01, 0.1, 1.0,10,20μg/ml respectively acted on Spc-a1 cells for 72 hours . [Results] The inhibition rates with concentration of 10μg/ml and 20μg/ml were 6.264%,7.78% respectively. Significant positive correlation was showed between inhibition of sandostatin on Spc-al cells and the dosage of sandostatin （P=0.001）. The rates of apoptosis of Spc-al cells induced by sandostatin with concentration of 0.01μg/ml, 0.1 μg/ml, 1.0 μg/ml were 1.89%,3.56%,4.44% respectively. [Conclusions] Sandostatin can inhibite the growth of lung adenocarcinoma cell line Spc-al in vitro.

关 键 词：善得定 肺腺癌 凋亡 抑制作用 

分 类 号：R73-3[医药卫生—肿瘤]