醛固酮合成酶基因多态性与小动脉顺应性的研究  

Relationship between the aldosterone synthase (CYP11B2)-344C/T polymorphism and small artery compliance

作　　者：李新立[1] 梁立荣[1] 倪春辉[1] 王震震[1] 李爱萍[1] 廖静[1] 周建伟[1] 曹克将[1] 黄峻[1] 

机构地区：[1]南京医科大学第一附属医院心内科,210029

出　　处：《中华心血管病杂志》2005年第7期599-602,共4页Chinese Journal of Cardiology

基　　金：江苏省自然科学基金项目(BK2002029)

摘　　要：目的探讨醛固酮合成酶(CYP11B2)基因-344C/T多态性与小动脉顺应性(C2)的关系及其临床意义.方法 (1) 用CVProfilor DO-2020动脉脉搏分析仪测量大小动脉顺应性,共224例,其中C2异常组123例,对照组101例.(2) 用多聚酶链反应-限制性片段长度多态性分析方法检测CYP11B2 基因-344C/T 多态性. 结果 (1) C2异常组TT基因型、T等位基因频率均高于对照组,但差异无统计学意义(55.3%比41.6 %,P>0.05, 75.6%比66.8%, P>0.05).(2) CC型与CT型合并分析,显示C2异常组TT型频率显著高于对照组(30.3%比18.8%,P<0.05).(3) 协方差分析显示,与CT/CC型比较,TT型者C2显著降低.(4) Logistic 回归分析表明,TT型是导致C2减退重要的基因型(P=0.043,OR=1.93 95%CI 1.02～3.63 ). 结论 CYP11B2基因-344C/T多态性与小动脉顺应性C2密切相关,TT型是C2减退的敏感基因型.Objective To investigate the relationship between the aldosterone synthase （ CYP11B2）-344C/T polymorphism and small artery compliance （C2）. Methods C2 was measured by CVProfilor DO2020 in 224 subjects, including 123 subjects with an abnormal C2 and 101 normal controls. Genotypes of CYP11B2 were determined by polymerase chain reaction-based restriction fragment length polymorphism analysis. Results The frequencies of the CYP11B TT genotype and T allele in subjects with an abnormal C2 were slightly higher than in normal controls, but the differences did not reach statistical significance （55.3% vs 41.6 %, P 〉 0.05, 75.6% vs 66. 8%, P 〉 0.05. However, when CT was combined with CC, the frequency of TT in subjects with an abnormal C2 was significantly higher than in normal controls （P 〈 0. 05 ）. By COANOVA, TT subjects had a lower C2 than CT and CC subjects （P 〈 0. 05）. Logistic regression analysis revealed that TT genotype was associated with abnormal C2 （ P = 0. 043, OR = 1.93 95% CI 1.02 - 3.63 ）. Conclusions The CYP11B-344C/T polymorphism is associated with small artery compliance, and TT subiects are susceptible to abnormality of small arterial compliance.

关 键 词：细胞色素P-450CYP11B2 基因 多态性 单核苷酸 小动脉 弹性 酶基因多态性 动脉顺应性 醛固酮合成酶 CYP11B2基因 限制性片段长度多态性分析 

分 类 号：R543[医药卫生—心血管疾病]