E-选择素A561C基因多态性对高血压的影响  

Effect of E-selectin A561C (S128R) polymorphism on blood pressure

作　　者：陈海翎[1] 华琦[1] 刘荣坤[1] 杨峥[1] 

机构地区：[1]首都医科大学宣武医院心内科,100053

出　　处：《中华心血管病杂志》2005年第7期603-607,共5页Chinese Journal of Cardiology

基　　金：首都医科大学发展基金重大课题(20022007)

摘　　要：目的探讨E-选择素A561C基因多态性对高血压病的影响.方法高血压病组347例,对照组315例.两组年龄、体重指数差异无统计学意义.对两组进行血压测量,采血检测一氧化氮、内皮素、血管紧张素Ⅰ、血管紧张素Ⅱ及醛固酮.聚合酶链反应-限制性片段长度多态性方法进行DNA多态性分析,电泳判断基因型并测序.结果检测到AA、AC、CC三种基因型.在高血压病组与对照组之间基因型构成比差异有统计学意义(P<0.05).在高血压病组AC-CC基因型和C等位基因频率(6.92%和4.76%)显著高于对照组(分别为3.17%, 2.22%),P值分别为0.029和0.013.C等位基因发生高血压病的风险性是A等位基因的2.197倍(OR=2.197, 95% CI为1.164～4.144).AC-CC基因型平均动脉压与舒张压显著高于AA基因型(P值分别为0.024和0.049); AC-CC基因型收缩压也有高于AA基因型的趋势,但差异无统计学意义.AC-CC基因型比AA型的空腹血糖、总胆固醇、低密度脂蛋白胆固醇更高(P=0.031,0.046,0.003),一氧化氮/内皮素比值更低(P=0.036).结论 E-选择素A561C基因多态性对高血压病有显著影响,AC-CC基因型和C等位基因发生高血压病风险较大,收缩压与平均动脉压较高.Objective To investigate the effect of E-selectin A561C （S128R） polymorphism on blood pressure. Methods 347 essential hypertensive patients （male 163 and female 184, age 46. 08 ± 12. 49 y, and body mass index 26. 13 ± 3.14 kg/m^2 ） and 315 normal controls （ male 93 and female 222, age 42. 21 ± 14. 67 y, and body mass index 25.66 ± 3.19 kg/m^2 ） were genotyped by polymerase chain reaction-restriction fragment length polymorphism （ PCR-RFLP）. Phenotypic measurements included blood pressure, blood glucose, serum triglycerides, serum total, high-density lipoprotein （HDL） and low-density lipoprotein （LDL） cholesterol, serum uric acid, blood urea nitrogen, nitric oxide, endothelin, angiotensin Ⅰ, and Ⅱ and plasma aldosterone. Results The frequencies of the AC and CC genotypes （6.92%） and C allele （4.76%） were significantly （P = 0. 029 and 0. 013 ） higher in hypertensive patients than normal controls （3.17% and 2. 22% ）. The relative risk of hypertension associated with the C allele was 2. 197 （95% CI： 1. 164-4. 144）. Both diastolic blood pressure and mean arterial pressure were higher in C allele carriers than AA subjects （ P = 0. 049 and 0. 024 ）. Furthermore, C allele carders, compared with AA subjects, had higher concentrations of blood glucose, and total and LDL cholesterol （ P = 0. 031, 0. 046, and 0. 003 ） and lower concentrations of nitric oxide （P = 0. 036 ）. Conclusion The E-selectin A561C （S128R） polymorphism might affect blood pressure in Chinese.

关 键 词：高血压 E-选择素 基因 多态性 单核苷酸 A561C基因多态性 高血压病 聚合酶链反应-限制性片段长度多态性方法 等位基因频率 低密度脂蛋白胆固醇 AA基因型 

分 类 号：R544.1[医药卫生—心血管疾病]