肝癌组织中TGF-β1、TGF-β1 RⅡ和NF-κB的表达  

Expression of TGF-β1, TGF-β1RII and NF-κB in hepatocellular carcinoma

作　　者：缪林[1] 张锁林[2] 季国忠[1] 范志宁[1] 刘政[1] 张平[1] 杨春[1] 

机构地区：[1]南京医科大学第二附属医院消化科,江苏省南京市210011 [2]江苏省丹阳市人民医院消化科,江苏省丹阳市212300

出　　处：《世界华人消化杂志》2005年第14期1663-1666,共4页World Chinese Journal of Digestology

基　　金：江苏省教育厅自然科学基金资助;No.02KJD320023~~

摘　　要：目的:探讨转化生长因子β1(TGF-β1)、转化生长因子β1Ⅱ型受体(TGF-β1R Ⅱ)、核转录因子-κB(NF-κB) 在肝细胞癌(HCC)中表达. 方法:在30例肝癌组织和癌旁肝组织中,用免疫组化技术.分别检测TGF-β1 TGF-β1R Ⅱ及NF-κB蛋白的表达;用原位杂交方法检测TGF—β1mRNA的表达, 以CD34标记血管内皮细胞,观察TGF-β1及TGF—β1R Ⅱ蛋白与微血管密度(MVD)、TGF-β1蛋白与TGF- β1mRNA、NF-κB与TGF-β1的关系. 结果:肝癌组织TGF-β1mRNA平均光密度明显高于癌旁组织(0.1 043±0.035 vs 0.0 620±0.0 225,P<0.01)、TGF—β1蛋白平均光密度表达明显高于癌旁组织(0.0725±0.0102 vs 0.0442±0.0 103,P<0.01);肝癌组织TGF-β1RⅡ蛋白平均光密度表达明显低于癌旁组织(0.0 402±0.0 113 vs 0.0 669±0.0 157,P<0.01); 肝癌组织NF-κB蛋白表达均明显高于癌旁组织(0.0 723±0.0 210vs 0.0 305±0.0 116,P<0.01),肝癌组织MVD明显高于癌旁组织(31.23±9.25 vs 4.24±2.10.P<0.01),肝癌组织TGF-β1蛋白阳性表达与MVD 呈正相关(t=3.25,P<0.01);TGF-β1 mRNA与TGF-β1 蛋白呈正相关(X2=8.21,P<0.01);NF-κB蛋白阳性表达与TGF-β1蛋白阳性表达呈正相关(X2=9.075,P<0.01); 而TGF-βⅡ蛋白与MVI)呈负相关. 结论:肝癌组织中TGF-β1基因的变化发生在多个水平,肝癌细胞中TGF-β1RⅡ、NF-κB蛋白表达异常,并与MVD相关,提示他们在肝细胞癌血管形成中发挥重要作用,NF-κB可能在介导TGF-β1的活化或产生中发挥一定的作用.AIM： To explore the expression of TGF-β1, TGF-β1 RⅡ and NF-κB in hepatocellular carcinoma （HCC） tiussues. METHODS： Immunohistochemistry was used to detect the expression of TGF-β1, TGF-βRⅡ and NF-κB protein in 30 HCC and the corresponding cancer-adjacent tissues. The expression of TGF-β1 mRNA was determined in 30 HCC and the corresponding cancer-adjacent tissues by in situ hybridization. Vascular endothelial cells were labeled with CD34 to observe the microvessel density （MVD）. Then the relationships between TGF-β1 protein and MVD, TGF-β1RⅡ protein and MVD, NF-κB and TGF-β1 protein as well as between TGF-β1 protein and mRNA were analyzed. RESULTS： The expression of TGF-β1 protein and mRNA in HCC tissues were significantly higher than those in the cancer-adjacent tissues （Mean optical density： 0.0 725 ±0.0 102 vs0.0 442±0.0 103, P〈0.01,0.1 043±0.0 350vs 0.0 620±0.0 225, P〈0.01 ）. The MVD in HCC tissues was also markedly higher than that in the cancer-adjacent tissues （31.23 ± 9.25 vs 4.24 ± 2.10, P〈0.01）. The level of NF-κB expression was significantly higher than that in the cancer-adjacent tissues （0.0 723±0.0 210 vs0.0 305 ± 0.0 116, P〈0.01）, while the level of TGF-βRⅡ was lower （0.0 402±0.0 113 vs0.0 669±0.0 157, P〈0.01）.Obviously positive relationships existed between TGF-β1 protein and MVD （t= 3.25, P〈0.01）, TGF-β1 mRNA and TGF-β1 protein （x^2 = 8.21, P〈0.01 ） as well as between NF-κB and TGF-β1 protein （x^2 = 9.075, P〈0.01）, but the expression of TGF-βRⅡprotein was negatively related to MVD. CONCLUSION： TGF-β1, TGF-β1RⅡ and N F-κB protein is abnormally expressed in HCC tissues and they are significantly related to MVD. They may play important roles in the angiogenesis of hepatocellular carcinoma. NF-κB may mediate the activation and production of TGF-β1.

关 键 词：肝癌 癌组织 TGF-Β1 TGF-β1RⅡ NF-ΚB 表达 

分 类 号：R735.7[医药卫生—肿瘤]