^(103)pd诱导犬胆管增殖平滑肌细胞凋亡及对相关基因的影响  

Effect of ^(103)pd radioactive stent on apoptosis of smooth muscle cells in bile duct and expression of caspase-3, Fas and bcl-2 gene in dogs

作　　者：何贵金[1] 吴荣[2] 高沁怡[3] 许书河[4] 高红[5] 姜维国[6] 蒋涛[5] 戴显伟[1] 马凯[1] 

机构地区：[1]中国医科大学第二临床学院普外科,辽宁省沈阳市110004 [2]中国医科大学第二临床学院放疗中心,辽宁省沈阳市110004 [3]中国医科大学第一临床学院同位素室,辽宁省沈阳市110001 [4]中国原子能研究院同位素研究室,北京市100050 [5]中国医科大学第二临床学院儿外实验室,辽宁省沈阳市110004 [6]中国医科大学第二临床学院病理科,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2005年第14期1721-1724,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨γ射线对胱冬肽酶-3(caspase-3),Fas和bcl-2基因在犬胆管壁增殖平滑肌细胞凋亡中的活性影响及意义. 方法:犬12只随机分2组,每组6只;建立胆管损伤后狭窄模型,在犬胆管内分别植入103pd(103钯)放射性支架和普通胆管支架,采用HE,TUNEL,RT—PCR, 免疫组化检测增殖内膜中胆管平滑肌细胞增殖、凋亡及相关caspase-3,bcl-2和Fas基因表达,在JEM- 1200EX电镜下观察凋亡细胞超微结构变化,并用计算机图像检测系统检2组胆管腔面积. 结果:103pd支架组犬胆管组织中caspase-3和Fas基因表达较普通支架组明显(0.44±0.09 vs 0.16±0.02, 83.33% vs 50.00%,P<0.05),出现明显增殖平滑肌细胞凋亡(87.90±7.96 vs 5.60±0.51,P<0.05)。肝外胆管无明显狭窄;而普通支架caspase-3和Fas基因低表达,胆管未出现增殖平滑肌细胞明显凋亡,而肝外胆管有明显狭窄.103pd支架组犬胆管组织中bcl-2基因表达较普通支架组弱(16.66%vs 83.33%,P<0.05), 且bcl-2基因低表达组犬胆管出现明显增殖平滑肌细胞凋亡,而且犬肝外胆管无明显狭窄;bcl-2基因高表达组犬胆管未出现增殖平滑肌细胞明显凋亡,而肝外胆管有明显狭窄. 结论:103pd放射性支架可以激活caspase-3和Fas基因, 促进犬胆管增殖平滑肌细胞凋亡;bcl-2基因表达水平与细胞对辐射的敏感性有关,103pd放射性支架通过降低bcl-2基因表达,促进犬胆管增殖平滑肌细胞凋亡,从而抑制犬肝外胆管狭窄.AIM： To investigate the expression of caspase-3, Fas and bcl-2 gene in γ-radiation-induced smooth muscle cells of bile duct. METHODS： Twelve dogs were randomly divided into ^103pd radioactive stent group （PRS, n = 6） and the control group （NC, n = 6）. Stenosis models of biliary duct after injury were established. ^103pd radioactive and normal stent were transplanted into the dogs of PRS and NC groups respectively. The T-radiation-induced apoptosis of the smooth muscle cells and the expression of Fas, bcl-2 gene were detected by TUNEL and immunohistochemistry respectively. The expression of caspase-3 mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR）.RESULTS： The caspase-3 and Fas gene were highly expressed （0.44 ± 0.09, 83.33% respectively） in dogs of PRS group, and significant apoptosis of muscle cells （87.90 ± 7.96） was observed. There was no marked stenosis in extrahepatic duct. However, caspase-3 and Fas were lowly expressed （0.16 ± 0.02, 50.00% respectively） in dogs of NC group, and no significant apoptosis of muscle cells （5.60 ± 0.51） was observed. Significant difference existed between dogs of PRS and NC group in caspase-3, Fas and bcl-2 expression and the apoptosis of muscle cells （P〈0.05）. Marked stenosis appeared in extrahepatic duct. The expression of bct-2 gene was significantly decreased in dogs of PRS group as compared with than of NC group （16.66% vs 83.33%, P〈0.05）.CONCLUSION： ^103pd radioactive stent can increase the expression of caspase-3 and Fas gene and promote the apoptosis of smooth muscle cells of bile duct. The level of bcl-2 expression is associated with the sensitivity of muscle cells to γ-radiation. ^103pd radioactive stent can facilitate the apoptosis of muscle cells by reducing bcl-2 expression, so as to prevent the stenosis of extrahepatic duct.

关 键 词：^103PD 诱导 胆管 增殖平滑肌 细胞凋亡 肝外胆管狭窄 

分 类 号：R575.6[医药卫生—消化系统]