E26转录因子-1在视网膜母细胞瘤中的表达及意义  

Expression of E26 transformation-specific-1 in retinoblastoma

作　　者：游志鹏[1] 姜德咏[2] 

机构地区：[1]江西医学院第二附属医院眼科,南昌330006 [2]中南大学湘雅二医院眼科

出　　处：《眼科研究》2005年第4期380-382,共3页Chinese Ophthalmic Research

摘　　要：目的探讨E26转录因子-1(Ets-1)在视网膜母细胞瘤(Rb)中的表达及其与肿瘤的血管化、浸润、转移的关系。方法利用原位杂交和免疫组织化学法分别检测Ets-1mRNA和蛋白在40例Rb中的表达。并以免疫组织化学法检测肿瘤中CD34的表达,以此计算肿瘤微血管密度的值。结果82·5%(33/40)的Rb中Ets-1mRNA呈阳性表达,Ets-1蛋白的阳性表达率为65%(26/40)。Ets-1主要表达于癌细胞和血管内皮细胞。Ets-1高表达组的微血管密度明显高于Ets-1低表达组(P<0·01)。其表达与组织学分级和预后有关。结论Ets-1可促进Rb的新生血管形成,与肿瘤的浸润和转移有关。Objective To study the expression of E26 transformation-specific-1 （ Ets-1） in retinoblastoma（Rb） and its effect on angiogenesis,tumor invasion and metastasis. Methods In situ hybridization and streptomycin avidin-biotinperoxidasec omplex SP were used to detect the mRNA and protein expression of Ets-1 in 40 cases of Rb. Expression of CD34 in the Rb was determined and microvascular density （MVD） was calculated by immunohistochemistry. Results Ets-1 mRNA and protein were highly expressed in Rb tumor cells with the positive rate 82.5% （33/40） and 65% （26/40） ,respectively. Ets-1 mRNA was over-expressed in mainly cell plasma and Ets-1 protein was in cell plasma and nucleus in both Rb cells and vessel endothelial cells with a positive correlation to tumor staging. The microvessel density was significantly higher in Ets-1 highly expressed group than in the low expressed group （ P 〈0. 01 ）. The expression of Ets-1 was significant correlated with pathologic grade and prognosis. Conclusion Ets-1 has a correlation with tumor invasion and metastasis and can promote angiogenesis in Rb. The detect of Ets-1 may be a reference indicator for the angiogenesis and invasion of Rb.

关 键 词：视网膜母细胞瘤 E26转录因子-1 新生血管 免疫组织化学 

分 类 号：R739.7[医药卫生—肿瘤]