结直肠癌组织iNOS与eNOS的组织芯片技术检测及其与血管生成关系  

EXPRESSION OF iNOS AND eNOS IN COLORECTAL CARCINOMA AND ITS RELATION TO ANGIOGENESIS

作　　者：崔琳[1] 江晨[1] 纪萍[1] 于建宪[1] 

机构地区：[1]青岛市市立医院病理科,山东青岛266011

出　　处：《齐鲁医学杂志》2005年第3期203-205,共3页Medical Journal of Qilu

基　　金：青岛市科技局资助项目(031NY142)

摘　　要：①目的探讨诱导型一氧化氮合酶(iNOS)、内皮型一氧化氮合酶(eNOS)两种血管生成因子在结直肠癌中表达及其与临床病理特征和微血管密度(MVD)的关系。②方法制作78例结直肠癌组织及非肿瘤结直肠黏膜组织芯片,用免疫组化技术检测其iNOS、eNOS的表达。③结果iNOS在结直肠癌表达阳性率高于非肿瘤结直肠黏膜(χ2=43.166,P<0.001),eNOS在结直肠癌表达阳性率低于非肿瘤结直肠黏膜(χ2=11.354,P<0.001)。结直肠癌组织iNOS表达阳性率随分化程度降低而降低,随浸润深度的增加而增加,差异均有显著性(χ2=18.141、4.748,P<0.01);eNOS阳性率在不同临床病理特征病人之间的差异无统计学意义;iNOS阳性组的MVD值高于阴性组,差异有统计学意义(t=2.327,P<0.05);eNOS阳性组的MVD值与阴性组之间的差异无统计学意义。④结论检测iNOS在结直肠癌中的表达对判断结直肠癌的恶性程度有价值;抑制iNOS在结直肠癌中表达可能成为抑制结直肠癌血管生成的途径。应用组织芯片大规模高效检测临床组织样本是可行的,具有快速、方便、经济、准确的特点。Objective To discuss the expression of iNOS and eNOS in colorectal carcinoma and its relation with angiogenesis. Methods The tissue microarray was made up of 78 cases of colorectal carcinoma. The expression of iNOS and eNOS was detected by immunohistochemistry. Results The positive rate of iNOS in colorectal carcinoma was higher than that in natural large intestine mucous membrane （x^2=43. 166, P〈0. 001 ）. The positive rate of eNOS in colorectal carcinoma was lower than that in natural large intestine mucous membrane （x^2= 11. 354, P〈0. 001）. The positive rate of iNOS would fall along with differentiation falling, and with invasive depth （x^2=18. 141,4.748;P〈0. 01）. The MVD of iNOS positive group was higher than that of negative group （t=2. 327, P〈0. 05）. The MVD between different eNOS groups had no difference. Conclusion Detecting the expression of iNOS in colorectal carcinoma is valuable to estimate the biological character of colorectal carcinoma. Inhibiting the expression of iNOS might be an approach to inhibit angiogenesis in the carcinoma. It is feasible to detect a lot of samples efficiently by using tissue mieroarray, which is fast, convenient, economical and precise.

关 键 词：一氧化氮合酶 结直肠肿瘤 新生血管化 病理性 组织芯片 

分 类 号：R735.34[医药卫生—肿瘤]