胃癌组织中HGF/c-Met的组织芯片检测及其与肿瘤血管生成关系  

EXPRESSION OF HGF/c-Met IN GASTRIC CARCINOMA AND ITS SELATIONSHIP WITH ANGIOGENESIS ON TISSUE MICROARRAYS

作　　者：江晨[1] 崔琳[1] 纪萍[1] 于建宪[1] 

机构地区：[1]青岛市市立医院病理科,山东,青岛,266011

出　　处：《齐鲁医学杂志》2005年第3期211-213,216,共4页Medical Journal of Qilu

基　　金：青岛市科技局资助项目[031NY142]

摘　　要：①目的探讨胃癌组织中肝细胞生长因子(HGF)及其受体(cMet)的表达与微血管密度(MVD)的关系及其意义。②方法应用组织芯片技术结合免疫组织化学SP法对88例胃癌组织及相应癌旁胃组织中HGF、cMet的表达及以CD105为标记的MVD进行观察。③结果cMet在肿瘤组织与癌旁胃组织中的表达差异有显著性(χ2=59.9,P<0.001);HGF、cMet在不同组织分化、分期胃癌的表达差异无显著性;在有无淋巴结转移、远处转移胃癌组织之间表达差异有显著性(χ2=3.90～5.15,P<0.05);HGF、cMet高表达同MVD增强显著相关(t=12.82、9.38,P<0.01)。④结论HGF/cMet的表达在胃癌侵袭进展、肿瘤血管生成过程中起重要作用,为肿瘤治疗提供了可能的途径;组织芯片是平行、高效的病理学研究手段。Objective To study the relationship between hepatocyte growth factor（HGF）/c-Met and angiogenesis in gastric carcinoma. Methods The expression of HGF, c-Met and CD105 of 88 cases of gastric carcinoma and the adjacent tissues were detected by immunohistochemistry on tissue microarrays. Results The expression of c-Met in gastric carcinoma was significantly different from that of the adjacent tissues （X^2=59.9, P〈0. 001）. The expression of HGF/d-Met correlated with lymph node metastasis and distant metastasis, but not with histological differentiation and stages. The expression of MVD increased significantly with increased expression of HGF/c-Met （t=12.82, 9.38; P〈0.01）. Conclusion HGF/c-Met, which is important in the progress and angiogenesis of gastric carcinoma, may be a valuable way in tumor therapy. Tissue microarrays is parallel and efficient in pathological studies.

关 键 词：胃肿瘤 肝细胞生长因子 C-MET 组织芯片 

分 类 号：R730.21[医药卫生—肿瘤]