硝苯地平对人牙龈成纤维细胞内钙离子含量的影响  

The effect of nifedipine on the concentration of intracellular Ca^(2+) in gingival fibroblast

作　　者：蒋春梅[1] 孙卫斌[1] 倪杰[1] 胡凡[2] 

机构地区：[1]南京医科大学附属口腔医院,江苏南京210029 [2]南京医科大学激光共聚焦室

出　　处：《临床口腔医学杂志》2005年第8期453-455,共3页Journal of Clinical Stomatology

摘　　要：目的:探讨硝苯地平对牙龈成纤维细胞内Ca2+含量的影响。方法:体外培养人牙龈成纤维细胞,Flu-o-3染色,激光共聚焦显微镜下观察加入不同浓度硝苯地平后荧光强度的变化。结果:硝苯地平能抑制DHP类钙钙离子指示剂通道BayK8644引起的牙龈成纤维细胞内钙离子含量增加(P<0.05),不同浓度硝苯地平的作用之间没有剂量-效应关系。结论:硝苯地平引起牙龈增生可能与抑制牙龈成纤维细胞内钙离子含量有关。Objective： To investigate the effects of nifedipine on concentrations of Ca^2＋ in gingival fibroblast cells. Method：gingival fibrobl^t cells were culture in vitro, then dyed with Fluo-3. The changes of light density of cells under confocal laser microscopy were investigated. Result：Nifedipine can inhabit the increase of concentration of Ca^2＋ induced by Bay K8644, and this effect is independent to concentration of Nifedipine. Conclusion： Gingival hyperplasia induced by nifedipine may be dated with the inhibition of fibrotblasintracellular Ca^2＋ concentration.

关 键 词：硝苯地平 牙龈成纤维细胞 细胞内钙离子 

分 类 号：R780.2[医药卫生—口腔医学]