计算机辅助设计抗肿瘤凋亡基因livin的反义核酸  

Design of livin antisense oligonucleotides with computer

作　　者：张华东[1] 袁守军[1] 陈惠鹏[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《中国临床药理学与治疗学》2005年第7期750-754,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究利用计算机辅助设计抗肿瘤细胞凋亡基因livinmRNA的反义核酸。方法:利用RNAdraw和Sfold两种软件进行livinmRNA反义核酸的二级结构预测,并转染肿瘤细胞HeLa,诱导细胞凋亡。结果:用这种方法设计出5个反义核酸,它们能够有效地抑制肿瘤细胞HeLa的生长,促进其凋亡。结论:利用这两种计算机软件设计livinmRNA反义核酸是可行的,能够有效的促进细胞凋亡。AIM： Antisense oligonucleotides against livin were designed with computer software. METHODS： Antisense oligonucleotides were designed according to the secondary structure of livin mRNA which was simulated with RNAdraw and Sfold. And then these oligonucleotides were transfected into Hela cells for inducing apoptosis. RESULTS： Five antisense oligonucleotides were designed using RNAdraw and Sfold, and effectively induced Hela cell apoptosis. CONCLUSION： The approach is effective and feasible to design antisense oligonucleotides by means of calculation with two kinds of software.

关 键 词：MRNA二级结构 RNAdraw Sfold LIVIN 反义核酸 

分 类 号：R966[医药卫生—药理学]