黄芪注射液对腹膜透析相关腹膜间皮细胞TGF-β_1分泌与表达的影响  

Effects of Astragalus on secretion and mRNA expression of TGF-β_1 induced by peritoneal dialysis solution in cultured human peritoneal mesothelial cell

作　　者：徐海红[1] 张苗[2] 蒋春明[2] 

机构地区：[1]南京大学医学院 [2]南京大学医学院附属鼓楼医院肾科

出　　处：《中国临床药理学与治疗学》2005年第7期791-794,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:探讨黄芪对腹膜透析相关性腹膜纤维化的作用。方法:人腹膜间皮细胞(HPMC)做体外培养,第三代细胞用于实验。分5组观察,对照组为RP-MI1640;PDS(peritoneal dialysis solution,腹膜透析液)组为加入PDS;黄芪12、3、组为加入含黄芪注射液的PDS,黄芪终浓度分别为102、04、0 mg.ml-1。分别在24 h采用ELISA法检测细胞上清液中转移生长因子-1β(TGF-1β)的含量,采用逆转录多聚酶链式反应(RT-PCR)检测TGF-1βmRNA表达。结果:腹膜间皮细胞在PDS干预下分泌TGF-1β明显增加(P<0.05)。腹膜间皮细胞经含不同浓度黄芪注射液的PDS干预后,TGF-1β分泌与PDS组比较有显著下降(P<0.05)。不同浓度黄芪注射液组之间比较无显著差异(P>0.05)。腹膜间皮细胞在PDS干预下TGF-1βmRNA表达明显升高,比对照组上升62.43%,腹膜间皮细胞经含不同浓度黄芪注射液的PDS干预后,TGF-1βmRNA表达显著低于PDS组(P<0.01)。结论:商业性腹膜透析液可促进腹膜间皮细胞分泌和表达TGF-1β,黄芪可以部分抵消这一作用所致的腹膜透析相关性腹膜纤维化。AIM： To investigate the effects of Astragalus on peritoneal fibrosis correlating peritoneal dialysis. METHODS： Human peritoneal mesothelial cells （HPMC） were cultured in vitro. They were divided into five groups： RPMI1640 culture medium as control group, PDS（ peritoneal dialysis solution） containing 1.5% glucose as PDS group, PDS containing 1.5 % glucose and Astragalus 10 mg·ml^-1 as Astragalus group 1, PDS containing 1.5 % glucose and Astragalus 20 mg· ml^- 1 as Astragalus group 2 and PDS containing 1.5 % glucose and Astragalus 40 mg· ml^-1 as Astragalus group 3 for testing. The levels of TGF-β1 in supematant of the cell culture was detected by the method of ELISA and the mRNA expression of TGF-β1 were tested by the method of reverse transcriptase PCR （RT-PCR） at 24 hours. RESULTS： The level of TGF-β1 was significant lower in control group than that in PDS group （P 〈 0.05）. Marked lower level of TGF-β1 was found in Astragalus group 1, Astragalus group 2 and Astragalus group 3 compared with the PDS group （ P 〈0.05）. The mRNA expression of TGF-β1 increased significantly to 162.43% compared with the control group. The mRNA expression of TGF-β1 increased 23.52% in Astragalus group 1, 21.36% in Astragalus group 2 and 18.71% in Astragalus group 3, respectively, and significant difference were found when compared with those in PDS groups （ P 〈 0.01 ）. CONCLUSION： Astragalus inhibits TGF-β1 protein and mRNA over-expression induced by commercial PDS and plays a beneficial role in peritoneal fibrosis correlating peritoneal dialysis.

关 键 词：黄芪 腹膜透析 腹膜间皮细胞 细胞因子 腹膜纤维化 

分 类 号：R572.4[医药卫生—消化系统]