维生素E琥珀酸酯联合阿霉素对乳腺癌细胞株Bcap-37的生长抑制作用  

Inhibitory Effect of Vitamin E Succinate Combined with Adriamycin on the Growth ofHuman Breast Cancer Cell Line Bcap-37

作　　者：张伟[1] 张军初[1] 朱大乔[2] 徐昕昀[1] 张玲珍[1] 王强[1] 

机构地区：[1]上海第二军医大学长征医院,200433 [2]上海第二军医大学

出　　处：《实用癌症杂志》2005年第3期232-234,共3页The Practical Journal of Cancer

基　　金：上海市科委科技发展基金资助(项目编号024119105)

摘　　要：目的检测维生素E琥珀酸酯(VES)联合阿霉素(ADM)对乳腺癌细胞生长抑制作用。方法以VES联合ADM作用于人乳腺癌Bcap-37细胞24h和36h,VES浓度为5、10、20mg/L,ADM的浓度分别为0.25、1、2mg/L。采用MTT法测定对细胞增殖的抑制作用,以流式细胞仪分析药物联合作用前后乳腺癌细胞凋亡率的变化和对细胞表面Fas表达的影响。结果VES联合ADM对乳腺癌Bcap-37细胞具有显著的生长抑制和凋亡诱导作用。流式细胞仪分析细胞周期,Bcap-37细胞的自然凋亡率为0.7%,5mg/L和20mg/LVES作用24h后凋亡率分别为10.1%和19.2%,1mg/L和2mg/LADM作用24h后的凋亡率分别为16.0%和23.3%。5mg/LVES联合1mg/LADM以及20mg/LVES联合2mg/LADM作用24h后的凋亡率分别升高至31.1%和40.7%。VES联合ADM作用24h后Bcap-37细胞表面Fas表达增强。结论VES联合ADM对乳腺癌Bcap-37细胞具有显著的生长抑制作用,其机制可能与细胞表面Fas表达上调有关。Objective To investigate the inhibitory effect of vitamin E succinate（VES） combined with adriamycin（ADM） on the growth of human breast cancer cell line Bcap-37. Methods Bcap-37 human breast cancer cells were treated with VES（5mg/L,10 mg/L and 20 mg/L） and ADM（0.25mg/L, 1 mg/L and 2 mg/L） for 24 h and 36 h. The inhibitory effect on the growth was measured with MTT method. Cell cycle and cell surface Fas expression were analyzed with flow cytometry. Results The combination of VES with ADM had significant inhibitory effect on the growth of Bcap-37 human breast cancer cells. Flow cytometry showed the apoptouc rate of Bcap-37 was 0.7%. Apoptotic rates of cells,when treated with VES at the concentration of 5 mg/L and 20 mg/L and ADM at 1 mg/L and 2 mg/L,were 10.1% and 19.2%, 16.0% and 23.3% respectively,and increased to 31.1% and 40.7% when treated with 5 mg/L VES combine with 1 mg/L ADM and 20 mg/L VES combine with 2 mg/L,respectively. Cell surface Fas expression increased after being treated with VES combined with ADM.Conclusion VES combined with ADM had significant inhibitory effect on the growth of Bcap-37 human breast cancer cells. The mechanism may relate to the upregulated expression of Fas on the surface of cancer cells.

关 键 词：维生素E琥珀酸酯 阿霉素 乳腺癌 Bcap-37细胞株 

分 类 号：R737.25[医药卫生—肿瘤]