去磷酸化RB蛋白表达对阿霉素诱导MCF-7/S细胞凋亡的影响  

The Effect of Adriamycin on Cell Apoptosis and the Expression ofDephosphorylated RB Protein in MCF-7/S Cell Line

作　　者：王文武[1] 姜浩[2] 樊光华[3] 

机构地区：[1]南京军区福州总医院,421001 [2]南华大学附属第一医院,330029 [3]江西省肿瘤医院,350025

出　　处：《实用癌症杂志》2005年第3期235-237,共3页The Practical Journal of Cancer

基　　金：湖南省卫生厅科研课题基金资助(2001-Y58)

摘　　要：目的检测经阿霉素(ADR)处理的人乳腺癌MCF-7/S细胞的凋亡率(AR)及去磷酸化RB蛋白表达的变化,以探讨ADR诱导细胞凋亡的可能机理。方法将体外培养的MCF-7/S细胞分为实验组(以不同浓度ADR处理细胞)及对照组(以等体积的生理盐水处理细胞);应用MTT比色法检测ADR对MCF-7/S细胞的抑制率(IR);应用流式细胞术检测实验组和对照组细胞的AR;采用S-P免疫组化染色法检测ADR作用后去磷酸化RB蛋白表达的变化。结果ADR抑制MCF-7/S细胞增殖,呈剂量依赖性,IC50为0.128mg/L;0.25、2、5μg/mlADR处理的MCF-7/S细胞的AR分别为0.171、0.184、0.259,而对照组MCF-7/S细胞的AR为0.045,两者比较,有非常显著性差异(P<0.01);5μg/mlADR实验组MCF-7/S细胞的去磷酸化RB蛋白的表达量为986.8±207.4,而对照组为131.7±31.9,两者比较,有非常显著性差异(P<0.01);5μg/mlADR实验组MCF-7/S细胞的AR与其去磷酸化RB蛋白的表达量呈正相关(γ=0.998,P=0.037)。结论ADR能抑制MCF-7/S细胞增殖并诱导其凋亡,其机理可能与去磷酸化RB蛋白表达水平上调有关。Objective To detect the changes of apoptosis rate（AR） and dephosphorylated RB protein expression in MCF-7/S cell line treated by adriamycin（ADR）,and to investigate its potential mechanism. Methods MCF-7/S cells cultured in vitro were randomly divided into experimental group and control group;Cell growth inhibition rate（IR） were examined by MTT colorimetric assay;The AR were determined by flow cytometry（FCM）;And the expression of dephosphorylated RB protein were detected with immunohistochemical method. Results MTT ashy revealed that ADR could inhibit the proliferation of MCF-7/S cells in a close-dependent manner, the 50 % inhibition concentration （IC50） value was 0. 128 mg/L ;The AR of MCF-7/S treated by 0.25,2,5μg/ml ADR was 0. 171,0. 184,0.259 respectively, which was significantly higher than that of control group（x^-=0. 045 ）（P〈0.01 ）;The expression of dephosphorylated RB protein in 5μg/ml ADR experimental group was 986.8±207.4,and that of control group was131.7±31.9（P〈0.01）;In 5μg/ml ADR experimental group, there was positive correlation between AR and the expression of dephosphorylated RB protein（γ=0.998, P=0.037）. Conclusion ADR exhibited growth inhibition effect and induced apoptosis on MCF-7/S cell line in vitro;Apoptosis of MCF-7/S cells induced by ADR may be related with up-regulating dephosphorylated RB protein.

关 键 词：去磷酸化RB蛋白 乳腺肿瘤 MCF-7/S细胞 凋亡 阿霉素 

分 类 号：R73-361[医药卫生—肿瘤]