塞来昔布诱导SGC-7901人胃癌细胞凋亡及其影响端粒酶活性的实验研究  

Study on the Effects of Apoptosis and Telomerase Activity by Celecoxib in SGC-7901 Human Gastric Cancer Cell Line

作　　者：亓玉琴[1] 司君利[1] 魏涛[1] 李文利[1] 贺远龙[1] 朱菊人[2] 

机构地区：[1]青岛大学附属青岛市立医院,250021 [2]山东省立医院,266011

出　　处：《实用癌症杂志》2005年第3期238-241,共4页The Practical Journal of Cancer

基　　金：青岛市卫生局科研基金资助项目(2004-wszd005)

摘　　要：目的探讨选择性环氧化酶-2(COX-2)抑制剂塞来昔布对SGC-7901人胃癌细胞生长、端粒酶活性,细胞凋亡及相关基因的影响,研究其抗肿瘤的作用机制。方法终浓度为100、200及300μmol/L的塞来昔布作用于SGC-7901人胃癌细胞后,采用MTT比色法测定细胞的生长抑制率,采用半定量-TRAP银染法检测端粒酶活性,流式细胞仪检测细胞周期、细胞凋亡率及bcl-2表达水平变化。结果不同浓度的塞来昔布对SGC-7901人胃癌细胞均有抑制作用,抑制率与对照组相比,差异均有显著性(P<0.05);流式细胞学检测到凋亡峰,细胞阻滞于G0/G1期。同时它也显著抑制胃癌细胞的端粒酶活性,其抑制作用呈时间-剂量依赖性,且端粒酶活性的降低与bcl-2表达水平下降有关。结论塞来昔布能抑制胃癌细胞的增殖,诱导细胞凋亡,降低端粒酶活性,其作用机制与bcl-2表达水平下降有关,此可能是COX-2抑制剂抗肿瘤的机制之一。Objective To study the effects of celecoxib,a selective COX-2 inhibitor,on cell proliferation,telomerase activity and apoptosis in SGC-7901 human gastric cancer cell line and to explore its anti-neoplasm mechanism. Methods MTT assay was used to determine cell proliferation after incubation in different concentrations（ 100,200,300 μmol/L） of celecoxib. Telomerase activity was detected by semi-TRAP assay, flow cytometry was adopted to examine apoptotic rate,cell cycle and bcl-2 level. Results Celecoxib inhibited significantly the growth of SGC-7901 human gastric cancer cells and elevated the inhibiting rate（P〈0.05） as compared with control groups. The apoptotic peak was detected with FCM and cells were blocked in G0/G1 stage. The telomerase activitywas significantly inhibited in dose and time dependent manner,and the inhibition of telomerase activity was associated with decrease of bcl-2 level. Conclusion COX-2 inhibitor celecoxib not only inhibited proliferation,but induced apoptosis and inhibited telomerase activity by partly decreasing bcl-2 level in SGC-7901 human gastric cancer cell line. The results indicate one of the mechanisms underlying the anti-cancer effect of COX-2 inhibitor.

关 键 词：塞来昔布 胃癌 端粒酶 凋亡 bcl-2芝蛋白 

分 类 号：R73-361[医药卫生—肿瘤]