灵芝多糖GLP的抗疱疹病毒作用机理  

Inhibition of Herpes Simplex Virus Infection by a GLP Isolated from Mycelium of Ganoderma lucidum

作　　者：刘静[1] 阳帆[1] 李珊珊[1] 王玉华[1] 杨小骏[1] 吴正辉[1] 郜金荣[1] 叶林柏[1] 

机构地区：[1]武汉大学生命科学学院病毒学国家重点实验室,湖北武汉430072

出　　处：《中国病毒学》2005年第4期362-365,共4页Virologica Sinica

摘　　要：从灵芝菌丝体中分离得到的多糖GLP具有抑制单纯疱疹病毒I型感染的作用,并对GLP抑制疱疹病毒复制的作用机制进行了初步探讨。GLP对Vero细胞的CC50值大于2000μg/mL,GLP在疱疹病毒感染细胞前、感染后和病毒感染细胞时加入到细胞悬液中的EC50分别为4.6、50和17μg/mL;而如果将GLP与细胞共同孵育后再用病毒去感染,其EC50为11μg/mL。同时,GLP抑制病毒感染的选择指数分别为435、40、118和182。如果在病毒感染细胞后加入GLP,在病毒的生物大分子的合成完成后而子代病毒粒子还未释放出来前去掉GLP,这时GLP对病毒感染就没有抑制作用。定量PCR试验进一步证明GLP发挥抑制疱疹病毒感染作用是通过阻断病毒感染细胞早期与细胞表明蛋白的吸附来实现的。We reported here that a Ganoderma lucidum polysaccharide （GLP）, one of the components extracted and purified from the liquid fermentation of mycelium of Ganoderma lucidum, was active against HSV-linfectionin Vero cells. TheCC50（50% cytotoxic concentration） value of GLP for Veto cells growth was more than 2000μg/mL The EC50 （500% effective concentration） of GLP for virus yield reduction assay was 4.6μg/mL and 11μg/mL when virus or Veto cells were pre-mixed with GLP, 17μg/mL when virus and GLP were added into the cell culture simultaneously, and 50μg/mL when GLP was added after virus infection. Meanwhile, the selective index（SI,ratio of CC50 to EC50） of GLP were more than 435, 182, 118 and 40, respectively. There was no significant antiviral activity to be detected when the GLP was presented in the culture after beginning infection and before progeny virus release. Quantitative real-time PCR of the infective supernatant further confirmed that the GLP blocked HSV-1 infection at early stages of the infection.

关 键 词：灵芝多糖 抗疱疹病毒 作用机制 

分 类 号：R285.5[医药卫生—中药学]