肝癌细胞BEL-7402中STAT3的组成性活性及对细胞迁移影响的研究  

THE STUDY ON THE STAT3 LABELED BY GFP AFFECTING THE MIGRATION OF BEL-7402 CELL LINE

作　　者：杨润琳[1] 潘飞燕[1] 袁俊[1] 郭仕英[1] 张宏霞[1] 赵东红[1] 李朝军[1] 

机构地区：[1]南京师范大学生命科学学院江苏省分子医学生物技术重点实验室,南京210097

出　　处：《实验生物学报》2005年第4期317-323,共7页Acta Biologiae Experimentalis Sinica

基　　金：江苏省科技项目资助(BK2000038)

摘　　要：信号转导和转录激活因子(STATs,Signaltransducersandactivatorsoftranscription)是JAK-STATs信号途径中一类重要的DNA结合蛋白,其异常表达和活化与多种肿瘤相关。本文研究发现,在肝癌细胞BEL-7402中STAT3具有组成性活性,能够持续地磷酸化,并转移入细胞核中行使功能。同时,我们成功构建了绿色荧光蛋白(GFP)标记的STAT3野生型(WT)/突变体(CYF)融合质粒,将其转入细胞,以荧光素酶报告系统验证了融合蛋白的活性;通过测定绿色荧光为标记的细胞迁移距离的方法证实,过表达野生型STAT3对肝癌细胞系BEL-7402细胞迁移有促进作用,而突变体STAT3能够降低癌细胞的迁移。Signal transducers and activators of transcription （STATs） are key DNA-binding proteins in JAK/STATs signal pathway. Aberrant expression and activation of STAT3 have been identified in many kinds of tumors. We report here that constitutive activation of STAT3 was present in BEL-7402 cells. We constructed the fusing genes of STAT3 （wild type/mutant） and GFP to study the function of constitutively activated STAT3 in BEL-7402 cells. By measuring the migration of the cells labeled by GFP-STAT3（WT/CYF）, we proved that overexpression of STAT3（WT） could augment the migration of BEL-7402 cells, while STAT3（CYF） could decrease the migration.

关 键 词：肝癌细胞 BEL-7402 STAT3 组成性活性 细胞迁移 信号转导因子 转录激活因子 

分 类 号：R735.7[医药卫生—肿瘤] Q753[医药卫生—临床医学]