Silica-supported Macroporous Chitosan Bead for Affinity Purification of Trypsin Inhibitor  

作　　者：Feng Na XI Jian Min WU Ming Ming LUAN 

机构地区：[1]Department of Chemistry, Zhejiang University, Hangzhou 310027

出　　处：《Chinese Chemical Letters》2005年第8期1089-1092,共4页中国化学快报（英文版）

基　　金：The work is partially supported by the National Natural Science Foundation of China(20277031).

摘　　要：Macroporous cross-linking chitosan layer coated on silica gel （CTS-SiO2） was prepared by phase inversion and polyethylene glycol （PEG）molecular imprinting methods. Formation of macroporous surtace was investigated by scanning electron microscopy （SEM） and BET analysis.The prepared bead was activated by reacting with 1,2-ethylene digiycidyl ether for introducing epoxy groups, and trypsin could be efficiently immobilized on the bead as a biospecific ligand.The bead bearing trypsin was employed to purify trypsin inhibitor （TIs） from egg white as affinity adsorbent.Macroporous cross-linking chitosan layer coated on silica gel （CTS-SiO2） was prepared by phase inversion and polyethylene glycol （PEG）molecular imprinting methods. Formation of macroporous surtace was investigated by scanning electron microscopy （SEM） and BET analysis.The prepared bead was activated by reacting with 1,2-ethylene digiycidyl ether for introducing epoxy groups, and trypsin could be efficiently immobilized on the bead as a biospecific ligand.The bead bearing trypsin was employed to purify trypsin inhibitor （TIs） from egg white as affinity adsorbent.

关 键 词：CHITOSAN SILICA trypsin inhibitor TRYPSIN affinity purification. 

分 类 号：O635.1[理学—高分子化学]