supported by the National Natural Science Foundation of China(No.22074148);the Beijing Municipal Natural Science Foundation,China(No.2242005);the Zhiyuan Science Foundation of Beijing Institute of Petrochemical Technology(BIPT),China(No.2024004);the Fund of the Beijing Institute of Petrochemical Technology Analytical Testing Research Center.
In this study,Pepsin@AuNPs(Pep@AuNPs)and Trypsin@AuNPs(Try@AuNPs)were synthesized by a microfluidic droplet system using Pepsin and Trypsin as protection reagents and NaOH as reducing reagents.Compared to the synthesi...
Pancreatitis is a common,life-threatening inflammatory disease of the exocrine pancreas.Its pathogenesis remains obscure,and no specific or effective treatment is available.Gallstones and alcohol excess are major etio...
This work was supported by the National Key Research and Development Program of China(2017YFC1600802);the National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South Xinjiang of China(No.FE202303).
During post-harvest storage of Cucumis sativus fruit,the application of trypsin treatment could increaseflavonoid compound levels and reduce oxidative damage.To investigate the mechanism of trypsin-inducedflavonoid bio-...
Supported by the Joint Construction Project of Henan Medical Science and Technology(No.LHGJ20220370);the Natural Science Foundation of Henan Province(No.232300420237).
AIM:To compare the safety and clinical outcomes of subconjunctival trypsin and dexamethasone(DEX)injections in the treatment of anterior chamber fibrin exudates in eyes with globe rupture following primary wound repai...
supported by the Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety(No.GDPKLAPPS2005);the School Level Talent Project of Lingnan Normal University(No.ZL2021009);the Science and Technology Planning Project of Zhanjiang(No.2020A01040);the Study on the Preparation of Active Polypeptide from the Processing Waste of White Shrimp and its Fatigue Resistance(No.2021E05022);the Scientific Research Capacity Improvement Project of the Key Construction Discipline of Guangdong Province(No.2022ZD JS079).
During postmortem storage,fluoride in Antarctic krill can be enriched in the muscle.Trypsin,as the most important digestive enzyme in Antarctic krill with a high activity in low temperature,plays a potential role in t...
the National Key Research and Development Program of China(2017YFC1600802).
Trypsin,a novel superoxide scavenger,significantly enhances the storage quality of Hylocereus undatus(H.undatus).To elucidate the preservation mechanism of trypsin on H.undatus,a widely targeted metabolomic analysis,a...
supported by the National Natural Science Foundation of China(21808020);the Applied Basic Research Program of Science&Technology Department of Sichuan Province(2018JY0151)。
Phlorizin(PHL)is a natural compound with strong antioxidant properties mainly found in apples.In this paper,the interaction mechanism of PHL with pepsin and trypsin was comparatively evaluated by computer simulation,f...
This work was financially supported by the Jiangsu Province Natural Science Fund for Distinguished Young Scholars(BK20200025);a grant from the Key Technologies R&D Program of Jiangsu Province(BE2019630);the China Postdoctoral Science Foundation(2021M691286).
Streptomyces griseus trypsin(SGT)is a bacteria-sourced trypsin that could be potentially applied to industrial insulin productions.However,SGT produced by microbial hosts displayed low catalytic efficiency and undesir...
supported by the Agence Nationale de la Recherche ANR(ANR-17-CE09-0032-01 to A.O.,M.P.-G.,and F.P.);NIST Office of Law Enforcement Standards,and a Marie Skłodowska-Curie/Freiburg Institute for Advanced Studies Senior Fellowship(both to J.J.K.);We thank F.Gisou van der Goot(Ecole Polytechnique Federale de Lausanne,Switzerland)for providing the pET22b-proAL plasmid containing the proaerolysin sequence.
Presently,proteins are identified by cleaving them with proteases,measuring the mass to charge ratio of the fragments with a mass spectrometer,and matching the fragments to segments within known proteins in databases....
supported by the National Natural Science Foundation of China (32170147,31900158)。
Animal-derived biological products, such as fetal bovine serum(FBS) and trypsin, are important supplements for scientific, pharmaceutical, and medical use. Although preventive guidelines and tests are implemented to r...