Endogenous Trypsin Triggers Free Fluoride Release from Antarctic Krill(Euphausia superba)Cuticle  

作　　者：PENG Yuanhuai JI Wei JI Hongwu HAN Zhiping ZHANG Shiqi WEN Yaping 

机构地区：[1]College of Food Science and Engineering,Lingnan Normal University,Zhanjiang 524048,China [2]College of Biological and Food Engineering,Guangdong University of Education,Guangzhou 510303,China [3]Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Guangdong Ocean University,Zhanjiang 524088,China [4]College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China [5]College of Continuing Education,Lingnan Normal University,Zhanjiang 524048,China

出　　处：《Journal of Ocean University of China》2024年第3期766-774,共9页中国海洋大学学报（英文版）

基　　金：supported by the Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety(No.GDPKLAPPS2005);the School Level Talent Project of Lingnan Normal University(No.ZL2021009);the Science and Technology Planning Project of Zhanjiang(No.2020A01040);the Study on the Preparation of Active Polypeptide from the Processing Waste of White Shrimp and its Fatigue Resistance(No.2021E05022);the Scientific Research Capacity Improvement Project of the Key Construction Discipline of Guangdong Province(No.2022ZD JS079).

摘　　要：During postmortem storage,fluoride in Antarctic krill can be enriched in the muscle.Trypsin,as the most important digestive enzyme in Antarctic krill with a high activity in low temperature,plays a potential role in this process.In this study,endogenous trypsin was purified and its properties were investigated.The involvement of trypsin in the generation of free fluoride from Antarctic krill cuticle was explored.Cuticle microstructure before and after hydrolysis was compared with scanning electron microscopy,and the ash samples of the hydrolyzed Antarctic krill cuticle were analyzed with X-ray diffraction,Fourier transform infrared spectroscopy,and electron dispersive spectroscopy,respectively.Mass spectrometry analysis and inhibition tests confirmed that the purified enzyme was endogenous trypsin.Results of the present study indicated that trypsin digestion caused the increases of the concentrations of both fluoride ions and free amino N simultaneously,while the protein coated on the cuticle surface was dissolved too.However,no compositional change was detected in the cuticle inorganic salts.These findings suggest that trypsin triggered free fluoride release from Antarctic krill cuticle.In addition,the kinetics of free fluoride release could be described by the equation C_(W)=(1-0.97^(-0.006t)-0.03e^(0.0558t))×337.53+10.50.The present study improved the understanding of the role of trypsin in free fluoride release from Antarctic krill cuticle,facilitating future studies aimed at reducing the fluoride content in krill protein during Antarctic krill processing.

关 键 词：Antarctic krill cuticle FLUORIDE TRYPSIN 

分 类 号：S917.4[农业科学—水产科学]