Trilobatin Acts as a Marker Metabolite Involved in Flavonoid Accumulation Regulated by CsWRKY28-MYC2 with Trypsin Activation in Cucumber (Cucumis sativus)  

作　　者：Enyan Chen Jingyu Jia Jiaju Sun Jie Wang Xinxin Chen Xin Li 

机构地区：[1]College of Food and Bioengineering,Henan University of Science and Technology,Luoyang,471023,China [2]Chinese Academy of Agricultural Sciences Cotton Research Institute,Anyang,455000,China [3]The National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South Xinjiang,Tarim University,Alar,843300,China [4]National Demonstration Center for Experimental Food Processing and Safety Education,Luoyang,471000,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第11期2837-2856,共20页国际实验植物学杂志（英文）

基　　金：This work was supported by the National Key Research and Development Program of China(2017YFC1600802);the National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South Xinjiang of China(No.FE202303).

摘　　要：During post-harvest storage of Cucumis sativus fruit,the application of trypsin treatment could increaseflavonoid compound levels and reduce oxidative damage.To investigate the mechanism of trypsin-inducedflavonoid bio-synthesis in C.sativus,we conducted a combined analysis of transcriptomics and widely targeted metabolomics.One hundred and seventy-five significantly different metabolites were obtained from metabolomics data.The kyoto encyclopedia of genes and genomes(KEGG)functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis pathway.By combining the results of the weighted gene co-expression network analysis(WGCNA)with the 130 upregulated phenylpropanoid metabolites,22 significantly upregulated phenylpropanoid metabolites were identified.Trilobatin was identified as the most prominent meta-bolite through cluster analysis and variable importance in projection(VIP)analysis.High performance liquid chro-matography(HPLC)experiments confirmed that trilobatin was the key metabolite induced by trypsin.The transcriptomic results showed that 1068 genes in the brown module of WGCNA were highly positively correlated withflavonoid biosynthesis.The gene set enrichment analysis(GSEA)identified leading edges in 4 key KEGG path-ways.Finally,combined with WGCNA and GSEA analysis results,35 core genes were obtained.The co-expression network of transcriptomics and metabolomics suggested that CsWRKY28 and CsMYC2 regulated the biosynthesis of trilobatin.The quantitative real-time polymerase chain reaction(RT-qPCR)and dual luciferase experiments con-firmed the activation effect of CsWRKY28 on CsMYC2 and downstream target genes.This study revealed the key transcription factors involved in the trypsin-controlled biosynthesis of trilobatin in C.sativus and provided a new theoretical basis for elucidating the molecular mechanism of trypsin preservation.

关 键 词：CsWRKY28 TRANSCRIPTOMICS trilobatin TRYPSIN widely targeted metabolomics 

分 类 号：Q94[生物学—植物学]