^(131)I标记抗VEGF单抗在荷瘤膀胱癌裸鼠体内的生物学分布  

The biodistribution of ^(131)I-labeled anti-VEGF monoclonal antibody in nude mice bearing human bladder cancer

作　　者：李忠俊[1] 滕本秀[1] 程绍钧[2] 张青[3] 

机构地区：[1]第三军医大学新桥医院输血科,重庆400037 [2]第三军医大学西南医院核医学科,重庆400038 [3]第三军医大学新桥医院核医学科,重庆400037

出　　处：《重庆医学》2005年第8期1125-1127,共3页Chongqing medicine

摘　　要：目的研究131I-sc-7269在荷人膀胱癌裸鼠体内的生物学分布特性。方法(1)Iodogen法标记抗-VEGFMcAb(sc-7269),SephadexG-50柱层析分离纯化制备131I-sc-7269,体外细胞结合分析检测标记抗体的免疫活性;(2)荷瘤裸鼠131I-sc-7269体内分布实验。结果(1)131I-sc-7269比活度为1.00MBq/μg(放射性浓度为18.30MBq/ml),放射性化学纯度为96.20%。体外131I-sc-7269与T24细胞结合率为59.12%;(2)体内分布实验显示131I-sc-7269在肿瘤组织中浓聚的峰时为注入标记抗体后96h,此时各组织T/NT比值为最高。结论131I-sc-7269动物模型肿瘤/膀胱放射性峰时出现在96h,比值高达6.44,为应用131I-sc-7269放射免疫显像诊断人膀胱癌的实验和临床研究奠定了一定的实验基础。Purpose To study the biodistribution of ^131I-anti VEGF McAb（sc-7269） in nude mice bearing human bladder cancer. Methods （1）sc-7269 was labeled with Na ^131I using the Iodogen method,subsequently separated the ^131I sc-7269 by sephadex G- 50 column chromatography. Then immune activity of ^131I-sc-7269 was identified using T24 cell binding analysis in vitro. （2）The biodistribution and radioimmunoimaging of ^131I-sc 7269 in nude mice bearing human bladder cancer were carried out. Results （1）The specific activity of ^131I-sc-7269 was 1.00MBq/μg, and radioactive concentration of ^131I-sc-7269 was 18.30MBq/ml. The radiochemical purity of ^131I-sc-7269 was 96.20%, the specific binding rate of ^131I-sc-7269 to T24 cells in vitro was 59.12%. （2）The biodistribution experiment showed that the peak time of ^131I sc-7269 concentrating in the xenograft was about 96h after injection, and at the same time the radioactive ratioes between tumor and various tissues were the highest. Conclusion The highest T/NT radioactive ratio of ^131I-sc-7269 in nude mice bearing human bladder cancer occuring at 96h after injection is 6.44. The present study shows that the maintaining immune activity of the ^131I-sc-7269 in vivo is suitable for RII.

关 键 词：膀胱癌 血管内皮生长因子 放射性碘 

分 类 号：R737.14[医药卫生—肿瘤]