大蒜素对大鼠心室肌细胞L-型钙通道的影响  

Effect of allitridi on calcium current in rat ventricular myocytes

作　　者：邓春玉[1] 庄宁宁[2] 单志新[1] 钱卫民[1] 阮小薇[1] 吴书林[1] 林曙光[1] 

机构地区：[1]广东省心血管病研究所心内科,广东省人民医院医学研究中心,广州510080 [2]清远市人民医院心内科

出　　处：《中药药理与临床》2005年第3期18-20,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(No:30300421);广东省人民医院基金课题资助项目(No:Y03041)

摘　　要：目的:探讨大蒜素对大鼠心室肌细胞L-型钙通道的作用。方法:用急性酶解法获得大鼠的单个心肌细胞,用标准的全细胞膜片钳技术记录钙通道电流。结果:5、50、250和500μmol/L大蒜素分别抑制钙电流4.4%、26.91%、38.06%、72.90%。250μmol/L大蒜素能使心肌细胞钙电流-电压曲线明显上移,峰电流密度从(7.17±0.65)pA/pF减少至(4.44±0.52)pA/pF(n=15,P<0.05),但激活电位、峰电位和翻转电位无明显改变(P>0.05)。大蒜素能使钙电流失活曲线明显左移(P<0.05)。大蒜素对钙电流激活曲线、失活再复活曲线和频率依赖性无明显影响(P>0.05)。结论:大蒜素呈浓度依赖性地抑制心肌细胞L-型钙通道。Objective： To determine the effect and mechanism of allitridi on l.-type calcium current （ ICa, L）. Methods： Whole-cell pacth clamp recording technique was used to record ICa, L in single cells isolated rat ventrieutar myocytes. Results： The nifedipine-sensitive L-type calcium current was recorded in the rat ventricular myocytes. Allitridi decreased the calcium channel current in a dose-dependent and voltage-dependent manner in ventricular myocytes of rats. 1. The current-voltage curve was shifted upwards, which active potential, peak potential and reverse potential had no significant change. 2. The inactivation curve was shifted to more negative potential, but the activation curve and the recovery curve were not altered. 3. Allitridi had no effect on frequent-dependency of calcium channel. Conclusion： Allitridi concentration- dependently decreased calcium channel current in ventricular myocytes of rat.

关 键 词：大蒜素 全细胞膜片钳技术 L-型钙电流 心肌细胞 心肌收缩 心肌细胞L-型钙通道 心室肌细胞 大鼠 细胞钙电流 单个心肌细胞 

分 类 号：R285.5[医药卫生—中药学]