化学计量学辅助紫外分光光度法同时测定洛美沙星和司帕沙星  

Studies on simultaneous determination of levofloxacin and sparfloxacin using UV-spectrophotometry assisted by chemometrics methods

作　　者：李树伟[1] 潘建章[2] 何艺[2] 王小红[2] 

机构地区：[1]四川大学轻纺与食品学院,四川成都610065 [2]四川师范大学化学学院,四川成都610066

出　　处：《化学研究与应用》2005年第4期471-473,共3页Chemical Research and Application

摘　　要：在pH=3.0的B—R缓冲溶液中,洛美沙星(LMX)和司帕沙星(SPFX)的最大吸收波长分别为286nm和296nm,两者吸收光谱严重重叠.本文用多元线性回归(MLR)、K-矩阵(AKC)、P-矩阵(CPA)等计算方法辅助紫外分光光度法,同时测定了模拟样中LMX和SPFX的含量。发现CPA法明显优于MLR和AKC,LMX和SPFX的平均回收率分别达99.5%和99.9%,相对标准偏差RSD分别为0.59%和0.85%.The absorption maxima of Levofloxaein and Sparttoxaein in pH 3B-R buffer solution lie at 286nm and 296nm, respectively, indicating strong overlapping absorption spectra. The above mentioned two components in simulated samples have been simultaneously determined using UV-Spectrophotometry assisted by some chemometrics methods such as Multiple Linear Regression （MLR） , AKC matrix method, and CPA matrix method. But only the CPA method can achieve satisfactory results.

关 键 词：洛美沙星 司帕沙星 紫外分光光度法 多元线性回归 K-矩阵 P-矩阵 

分 类 号：O657.32[理学—分析化学]