LC/MS/MS法同时测定卷烟主流烟气中4种TSNAs  

Simultaneous determination of four tobacco-specific nitrosamines in cigarette mainstream smoke by LC/MS/MS

作　　者：毛友安[1] 钟科军[1] 魏万之[2] 卢红兵[1] 魏新亮[1] 

机构地区：[1]常德卷烟厂技术中心,湖南常德415000 [2]湖南大学化学化工学院,湖南长沙410082

出　　处：《化学研究与应用》2005年第4期571-573,共3页Chemical Research and Application

摘　　要：A liquid chsomatographic-tandem mass spectrometric method was used to simultaneously determine four tobacco-specific nitrosamines in cigarette mainstream smoke.Cigarette smoke particulate,obtained under standardized machine smoking,was collected on a Cambridge filter pad.The particulate was co-extracted with citric acid-phosphate buffer and cyclohexane and further purified by solid-phase extraction.Analyte identification and quantification were performed using multiple reaction monitoring with one precursor ion and two product ions as identifiers and electrospray ionization in positive mode.The limits of detection were in the range of 0.05～0.24ng/mL using an injection volume of 20μL,linear range over 2.5～1000ng/mL,linear correlation coefficients above 0.998,and recoveries 90.2%～95.1%.A liquid chsomatographic-tandem mass spectrometric method was used to simultaneously determine four tobacco-specific nitrosamines in cigarette mainstream smoke. Cigarette smoke particulate, obtained under standardized machine smoking,was collected on a Cambridge filter pad. The particulate was co-extracted withcitric acid-phosphate buffer and cyclohexane and further purified by solid-phase extraction. Analyte identification and quantification were performed using multiple reaction monitoring with one precursor ion and two product ions as identifiers and electrospray ionization in positive mode. The limits of detection were in the range of 0. 05～0. 24ng/mL using an injection volume of 20μL, linear range over 2. 5～1000ng/mL, linear correlation coefficients above 0. 998 ，and recoveries 90. 2%～95. 1%.

关 键 词：LC/MS/MS 烟草特有亚硝胺 卷烟主流烟气 多反应监测 

分 类 号：O657.7[理学—分析化学]