转化生长因子-β及其受体在人类肾细胞癌中的表达  

Expression of transforming growth factor-β and its receptor in human renal cell carcinoma

作　　者：巴建明[1] 朱开元[1] 李慧云[1] 

机构地区：[1]同济大学附属第十人民医院肾内科,上海200072

出　　处：《上海医学》2005年第7期598-600,共3页Shanghai Medical Journal

摘　　要：目的探讨转化生长因子(TGF)-β及其受体(TGF-βR)的表达与肾细胞癌(RCC)的关系.方法采用逆转录聚合酶链反应(RT-PCR)及表达丰度定量法检测肾细胞癌组织和正常对照组中TGF-β1～3、TGF-βRⅠ～Ⅲ的mRNA表达.结果 RCC组TGF-β1～3表达水平均明显高于正常对照组(P<0.005),而TGF-β1更为显著;RCC组的TGF-βRⅡ表达水平明显低于正常对照组 (P<0.005);而TGF-βRⅠ、Ⅲ的表达水平略高于正常对照组,但差异无显著性(P>0.05).结论 RCC组织中TGF-β的表达较正常肾组织高,而TβRⅡ的表达则明显低于正常肾组织,由此降低癌细胞对TGF-β的敏感性,减弱TGF-β抑制肾癌细胞增殖的作用,对TGF-β、TβRⅡ表达水平的检测有助于RCC的诊断和预后判断;TβRⅠ、Ⅲ的表达水平可能与RCC无明显关系.Objective To appraise the gene expression and relationship of transforming growth factor（TGF）-β and TGF-β receptor in RCC. Methods TGF-β and TGF-βR mRNA were analyzed by RT-PCR in 18 RCC tissues and 18 normal renal tissues （as control） which were collected during operation. Results The TGF-β1～3 gene expression in RCC was higher （P〈0. 005） and the expression of the TGF-βRⅡ was lower （P〈0. 005）. But the gene expressions of TGF-βR Ⅰ and TGF-βR Ⅲ were slightly higher than those of normal but were with no significant difference （P〉0.05）. Conclusions Expression of TGF-β is increased and that of TβR Ⅱ is decreased in RCC. The decrease of TβR Ⅱ expression may be the cause of impaired sensitivity to TGF-β in RCC suggesting as a significant prognostic factor in patients with RCC. （Shanghai Med J,2005,28：598-600）.

关 键 词：转化生长因子-Β 转化生长因子-Β受体 肾细胞癌 基因表达 逆转录聚合酶链反应 受体 TGF-Β1 TGF-βRⅠ 正常对照组 TGF-βRⅡ 

分 类 号：R737.11[医药卫生—肿瘤]