肠毒素大肠杆菌耐热肠毒素与谷胱甘肽巯基转移酶融合蛋白的制备和耐热肠毒素抗体测定  

Construction of enterotoxigenic Escherichia coli heat-stable enterotoxin fusion protein with glutathione S-transferase and detection of antibody against heat-stable enterotoxin

作　　者：郑继平[1] 刘向昕[1] 王令春[1] 王芃[1] 李淑琴[1] 张兆山[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《中国免疫学杂志》2005年第8期617-618,共2页Chinese Journal of Immunology

摘　　要：目的:弥补ST的弱抗原性,实现ST抗体测定。方法:采用生物工程技术,融合表达GST和带有前导序列的突变耐热肠毒素proSTm,制备ST融合蛋白,测定ST抗体。结果:测到了抗ST的血清IgG和黏膜IgA。结论:融合蛋白GST/proSTm能有效提高ST的抗原活性,可直接用于ST抗体测定。Objective：To detection antibody against heat-stable enterotoxin by fusion protein. Methods：Mutant heat-stable enterotoxin precursor gene was ligated in vector pGEX-4T-2 to inductively express as a fusion protein GST/proSTm with glutathione S-transferase（GST）. To investigate the antigenic action, serum and fecal antibodies against heat-stable enterotoxin was detected with this fusion protein. Results：The fusion protein was a about 32 kD protein. All the samples contain the antibody against ST.Conclusion：Such strategy was a promising method to detect antibody against heat-stable enterotoxin.

关 键 词：耐热肠毒素 谷胱甘肽巯基转移酶 融合蛋白 抗体测定 

分 类 号：R373[医药卫生—病原生物学]