TOCP对DFP在鸡脊髓神经细胞膜特异结合的影响  

Effect of tri-o-cresyl phosphate pretreatment on high-affinity bind of diisopropyfluorophosphate to membrane-bound proteins of chicken spinal cord

作　　者：朴丰源[1] 陈敏[1] 戴红[1] 刘启贵[1] 马宁[2] 仲来福[1] 山内彻 横山和仁 

机构地区：[1]大连医科大学,辽宁大连116027 [2]日本国立三重大学医学部

出　　处：《大连医科大学学报》2005年第4期249-252,共4页Journal of Dalian Medical University

基　　金：日本文部科学省科研基金(13576019)

摘　　要：[目的]调查〔3H〕DFP在各脊髓段神经细胞膜上的特异结合。[方法]鸡曝露于Tri-o-cre-sylphosphate(TOCP)后,隔6、24、48h处死,取颈、胸、腰段脊髓,称重后制成匀浆。经超速离心,获得膜蛋白。在脊髓膜蛋白中加入8nM的〔3H〕DFP,或加入80nM的非标记DFP后又加入8nM的〔3H〕DFP,然后培养1h。用nitrocellulosefilter进行快速真空滤过,用Tris-HCl-NaCl液对滤纸冲洗,再加5mL的Aquasol-2后,计数〔3H〕DFP的结合量。[结果]对照组鸡的颈、胸、腰椎段脊髓神经细胞膜上的〔3H〕DFP特异结合量分别为832.0、857.0、864.0fmol/mg,TOCP曝露组为273.5、243.52、71.5fmol/mg。TOCP曝露组各段脊髓神经细胞膜上的〔3H〕DFP特异结合量显著低于对照组,而各段脊髓神经细胞膜之间〔3H〕DFP的特异结合量无显著性差异。随着TOCP曝露后的时间的推移,各段脊髓神经细胞膜上的〔3H〕DFP特异结合量逐渐增高,提示迟发性神经毒性有机磷化合物的特异结合膜蛋白是较均匀地分布在整个脊髓神经细胞膜上。在这些脊髓神经细胞膜上的特异性结合部位,TOCP和DFP之间有竞争性抑制作用。[结论]脊髓神经细胞膜上的特异性结合膜蛋白可能与有机磷化合物的迟发性神经毒性诱发有关。[Objective] To investigate high- affinity bind of the delayed neurotoxic organophosphate to membrane- bound proteins from different sections of the chicken spinal cord. [ Methods] The chickens were sacrificed 6, 24 and 48 hrs after Tri-o-cresyl phosphate （TOCP） treatment and spinal cords were taken out.Membrane-bound proteins were prepared and the protein sites that labeled with a low concentration of[^3H] DFP were defined as the high- affinity binding sites. [ Results] Amount of[^3H] DFP binding to membranebound proteins of nerve cells in the cervical, thoracic and lumbar vertebrae was 832.0,857.0,864.0 fmol/mg in controls, respectively, and 273.5,243.5,271.5 fmol/mg in TOCP group. There was significant difference between groups and not between different sections of spinal cord in the same group. These results showed that TOCP metabolites interacted with the DFP binding sites and that membrane - bound proteins with DFP were relatively evenly distributed in chicken spinal cord membranes.[ Conclusions] It indicates that the membranebound proteins may be associated with the organophosphate- induced delayed neurotoxicity （OPIDN）.

关 键 词：迟发性神经毒性 有机磷化合物 鸡 脊髓神经细胞膜 tri-o-cresyl phosphate(TOCP) diisopropylfluorophosphate(DFP) 

分 类 号：R363[医药卫生—病理学]