豇豆胰蛋白酶抑制剂(CpTI)基因转化欧美杨的研究  

Transformation of Populus Euramericana with CpTI Gene

作　　者：赵强[1] 赵志文[1] 张廷婷[1] 崔德才[1] 王斌[2] 

机构地区：[1]山东农业大学生命科学学院,泰安271018 [2]中国科学院遗传与发育研究所,北京100101

出　　处：《生物技术通报》2005年第4期54-58,共5页Biotechnology Bulletin

摘　　要：本研究建立了欧美107杨的高频再生体系,用改良的根癌农杆菌介导法将豇豆胰蛋白酶抑制剂(CpTI)基因导入107杨中。经过严格的卡那霉素(Km)筛选,得到了Km抗性(Kmr)植株。对部分Kmr植株进行PCR及PCR-Southern杂交鉴定,证实CpTI基因已整合进杨树基因组中。转基因植株的饲虫实验表明,其中部分株系的叶片可在一定程度上抑制扁刺蛾幼虫的生长。The high frequency regeneration system of Populus euramericana 107 was established in this study, The Cowpea trypsin inhibitor （CpTI）gene was introduced by modified Agrobacterium tumefaciens mediated method into 107. Kanamycin resistant （Km^r）plantlets were obtained by strict screening selective condition added kanamycin. PCR and PCR-Southern tests from part of Km^r plantlets showed that CpTI gene has been integrated into Populus euramericana genome, And some transgenic plants could inhibit the development of larvae to some extent.

关 键 词：豇豆 胰蛋白酶抑制剂 CPTI 基因转化 欧美杨 转基因植株 

分 类 号：S792.119[农业科学—林木遗传育种]