混合表面活性剂介质中微量核酸的光谱测定  

Spectroscopic Analysis of Nucleic Acids in Mixed Surfactant

作　　者：陈莉华[1] 尹红[1] 杨朝霞[1] 沈含熙[2] 刘六战[2] 

机构地区：[1]吉首大学化学化工学院,湖南吉首416000 [2]南开大学化学学院,天津300071

出　　处：《化学世界》2005年第8期471-474,共4页Chemical World

基　　金：国家自然科学基金(20075012);湖南省教委自科基金(02C313)资助项目。

摘　　要：健那绿(Janus Green B,JGB)与核酸形成离子性缔合物增溶于混合非离子性表面活性剂中,导致吸光度降低,降低程度与加入的核酸浓度成线性关系,在最佳反应条件下,小牛胸腺DNA(ctDNA)、鱼精子DNA(fsDNA)及酵母RNA(yRNA)的线性范围分别为0.0～4.2μg/mL,0.0～2.4 μg/mL及0.0～1.9μg/mL,检测限分别为ctDNA:57 ng/mL,fsDNA:96 ng/mL及yRNA:42 ng/mL,用双倒数直线方程求出各种核酸与JGB的结合常数,均为104数量级,本法已用于实际样品分析.Through dissolving and stability function of mixed-surfactant, Janus Green B （JGB） could react with DNA （RNA） via static electronic force, leading to decrease in its absorbility. Under optimal conditions, a linear relationship was obtained between the decreased absorbility and the concentration of nucleic acids. The linear ranges were 0.0 - 4.2 μg/mL for calf thymus （ct DNA）, 0.0-4.2 μg/mL for fish sperm （is DNA） and 0.0 - 1.9 μg/mL for yeast RNA （yRNA） . The detection limits were 57 ng/mL for ct DNA, 96 ng/mL for fs DNA and 42 ng/mL for y DNA. The binding constants of nucleic acids-JGB were calculated and nucleic acids in chicken blood extraction were analyzed with satisfactory results.

关 键 词：核酸 光谱法 表面活性剂 

分 类 号：TQ420.7[化学工程]