当归补血汤煎剂中醋酸乙酯部位的主要化学成分分析  

Study on the Principal Chemical Composition of Ethyl Acetate Part in Angelicae Sinensis Decoction for Supplementing Blood Apozem

作　　者：黄月纯[1] 黄水清[2] 刘东辉[2] 魏刚[1] 

机构地区：[1]广州中医药大学附属第一医院,广州市510405 [2]广州中医药大学,广州市510405

出　　处：《中国药房》2005年第16期1275-1277,共3页China Pharmacy

基　　金：广东省中医药管理局资助课题(103025)

摘　　要：目的:分析当归补血汤煎剂中醋酸乙酯部位主要化学成分。方法:采用高效液相色谱法,色谱柱为HypersilODS,流动相为甲醇-0.5%冰醋酸(梯度洗脱),检测波长为280nm,流速为1ml/min,柱温为室温。结果:当归补血汤煎剂中醋酸乙酯部位主要有7个特征峰,其中6个峰来自黄芪的异黄酮,1个峰来自当归的阿魏酸。结论:当归补血汤煎剂中的主要特征峰为黄芪、当归煎剂峰的叠加,而未产生明显的其它组分峰。OBJECTIVE：To study the chief chemical components of the ethyl acetate part of Angelicae Sinensis Decoction for Supplementing Blood Apozem.METHODS： The HPLC was adopted in which Hypersil ODS was taken as the chromatographic column, the Methanol- 0.5% glacial acetic acid was taken as the mobile phase（gradient elution）, the^detective wavelength was 280nm, the flow rate was lml/min and with the column temperature set at room temperature.RESULTS： There were 7 characteristic peaks in the ethyl acetate part of Angelicae Sinensis Decoction for Supplementing Blood Decoction, 6 of which come from isoflavone of Radix Astragali and 1 come from ferulic acid of Angelicae Sinensis.CONCLUSION： The chief characteristic peak of the ethyl acetate part of Angelicae Sinensis Decoction for Supplementing Blood Apozem was the building up of Radix Astragali peak and angelicae sinensis peak,no other significant composition peaks were emerged.

关 键 词：当归补血汤 黄芪 当归 异黄酮 阿魏酸 高效液相色谱法 

分 类 号：R927.1[医药卫生—药学]