金丝桃素光动力作用诱导人鼻咽癌细胞凋亡  

Study of hypericin-mediated photodynamic therapy on human nasopharyngeal carcinoma cells

作　　者：许川山[1,2] 梁荣能[2] 

机构地区：[1]重庆医科大学超声影像学研究所,重庆400010 [2]香港浸会大学中医药学院教学部,中国香港

出　　处：《激光技术》2005年第4期395-397,共3页Laser Technology

基　　金：国家自然科学基金资助项目(30300468);全军医药卫生十五科研资助项目(01Q102)

摘　　要：为了观察中药金丝桃素光动力作用对鼻咽癌细胞凋亡的影响,应用膜联蛋白AnnexinV-PI双染结合流式细胞仪,分析了中药金丝桃素光动力作用后,人鼻咽癌细胞株CNE2细胞发生凋亡和继发性坏死的比率。中药金丝桃素光动力作用实验组人鼻咽癌细胞株CNE2细胞发生凋亡和继发性坏死的比率分别增加到53.08%和6.77%,且均显著高于单纯光照射组、单纯金丝桃素光敏剂处理组和假照射组(P<0.01),而单纯光照组、单纯金丝桃素光敏剂处理组和假照射组三对照组间无明显差异(P>0.05)。中药金丝桃素光动力作用能有效诱导人鼻咽癌细胞株CNE2细胞凋亡的发生,这也可能是金丝桃素光动力作用杀伤鼻咽癌的重要机制之一。To observe the effect of hypericin-mediated photodynamic therapy on apoptosis of human nasopharyngeal carcinoma cells, the apoptotie and secondary necrotic rate of the nasopharyageal carcinoma （NPC） CNE2 cells induced by hypericin-mediated photodynamic therapy was determined by flow cytometry with Annexin V-PI staining. The apoptotic and secondary necrotic rate of the NPC CNE2 cells after photodynamie therapy significantly increased to 53.08% and 6.77% , and they were higher than those of the controls respectively （P 〈 0.01 ） ,but there was no significance of the apoptotie and secondary necrotic rate between the controls （ P 〉 0.05 ）. Hyperiein-mediated photodynamie therapy might obviously trigger the NPC CNE2 cells into apoptosis, which might be one of the mechanisms of hyperiein-mediated photodynamie therapy on nasopharyngeal carcinoma。

关 键 词：金丝桃素 光动力治疗 鼻咽癌细胞 凋亡 

分 类 号：R730.57[医药卫生—肿瘤]