颞叶癫痫病灶内bax、fas、caspase-3蛋白的表达  

Expression of bax,fas and caspase-3 in human temporal lobe epilepsy

作　　者：尚伟[1] 毕建忠[1] 刘伟红[1] 孙琳[1] 来超[1] 王成伟[2] 庞琦[3] 

机构地区：[1]山东大学第二医院神经内科,济南250033 [2]山东大学第二医院神经外科,济南250033 [3]山东省立医院神经外科,济南250021

出　　处：《临床与实验病理学杂志》2005年第4期411-413,418,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：山东省自然科学基金(05200140)

摘　　要：目的探讨细胞凋亡调控基因bax、fas、caspase3在颞叶癫痫患者病灶内的表达及意义。方法应用免疫组化SP法检测24例颞叶癫痫患者手术切除病灶内凋亡相关基因bax、fas、caspase3的表达,同时采用光镜、电镜及原位末端标记(TUNEL)方法检测神经细胞凋亡的情况。结果bax蛋白在癫痫组与对照组中均轻微表达,两者间差异无显著性(P>0.05);fas蛋白在对照组中无表达,在癫痫组表达明显增强(P<0.001);caspase3在对照组中有轻微表达,在癫痫组表达明显增强(P<0.01)。光镜检查及TUNEL染色均未发现凋亡的神经细胞,但电镜检查的8例标本中有3例发现少量早期凋亡征象的神经元。结论癫痫患者脑内存在神经元凋亡现象。fas、caspase3基因在这一过程中可能发挥了重要作用。Purpose To explore the role of cell apoptosis regulatory genes of bax, fas and caspase-3 in the brain of human temporal lobe epilepsy. Methods The specimens were obtained from 24 patients of temporal lobe epilepsy. Neuron apoptosis was investigated by light and electron microscopy and TdT-mediated dUTP-biotin nick end labeling （TUNEL） method and immunohistochemistry for bax, fas and caspase-3. Results Compared with control group, Bax immunoreactivity was not increased in epilepsy group （ P〉0.05 ） ; Fas immunoreactivity was not found in control group; It significantly increased in epilepsy group（ P〈0.001 ）. Caspase-3 immunoreactivity was found in some neurons in control group, and it significantly increased in epilepsy group （ P〈0.01 ）. The evidence for neuron apoptosis was not found by microscopy, electron microscopy and TUNEL method in control group. But some early signs of neuroh apoptosis in 3 patients （3/8） were found by electron microscopy in epilepsy group. Conclusions Neuron apoptosis is involved in temporal lobe of human epilepsy. Fas and caspase-3 may play a role in this process.

关 键 词：癫痫 颞叶 细胞凋亡 BAX蛋白 FAS蛋白 CASPASE-3蛋白 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R329.25[医药卫生—临床医学]