Galectin-3、CK19和TPO表达在甲状腺良恶性肿瘤病理诊断中的价值  

Diagnostic value of the expression of galectin-3,CK19 and TPO in thyroid benign and malignant tumors

作　　者：郑璐滢[1] 朱建善[1] 王家东[2] 许雁萍[1] 

机构地区：[1]上海第二医科大学附属仁济医院病理科,上海200001 [2]上海第二医科大学附属仁济医院耳鼻喉科,上海200001

出　　处：《临床与实验病理学杂志》2005年第4期449-453,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的比较甲状腺病变中galectin3、CK19和甲状腺过氧化物酶(thyroidperoxidase,TPO)蛋白表达的差异,寻找有助于诊断良恶性肿瘤的标志物。方法采用免疫组化EnVision二步法检测134例甲状腺癌(123例乳头状癌、11例滤泡癌)、34例甲状腺腺瘤、20例结节性甲状腺肿和10例桥本甲状腺炎中galectin3、CK19和TPO蛋白的表达。结果Galectin3、CK19和TPO在甲状腺乳头状癌(包括主要的3个亚型)与良性病变中的表达差异有显著性(P<0.01),在乳头状癌各亚型间和各良性病变之间的表达差异均无显著性(P>0.05)。滤泡癌和良性病变中galectin3表达差异有显著性(P<0.01)。滤泡癌和腺瘤中CK19、TPO的表达差异有显著性(P<0.05,P<0.01)。结论甲状腺恶性肿瘤特别是乳头状癌中galectin3和CK19的表达有增高,而TPO表达缺失。3种蛋白分别是诊断甲状腺肿瘤特别是乳头状癌的有用标志物,联合使用结果更可靠。Purpose To compare the expression of galectin-3, CK19 and TPO in thyroid lesions for finding the valuable diagnostic markers to distinguish the benign lesions from the malignant tumors. Methods By immunochemical EnVision technique, the expression of galectin-3, CKI9 and TPO was detected in 134 cases of carcinomas （ including 123 papillary carcinomas and 11 ollicular carcinomas） , 34 follicular adenomas and 20 multinodular goiters and 10 Hashimoto＇s thyroiditis. Results Expression of galectin-3 ,CK19 and TPO were obviously different in the papillary carcinomas （ including the three main varieties ） and benign lesions （ P〈0.01 ）. There was no difference between each variety of papillary carcinomas and those of benign lesions （ P〉0.05 ）. There was significant difference between the follicular carcinomas and benign lesions concerning the expression of galectin-3 （P〈0.01）. The expression of the CK19 and TPO in follicular carcinomas both differed significantly from that in the adenomas （P〈0.05, P〈0.01）. Conclusions The expression of galectin-3 and CK19 is increased in malignant thyroid tumors, especially in papillary carcinomas, but loss of TPO. Combined application of immunohistochemical staining of galectin-3, CK19 and TPO is a more useful option to distinguish malignant tumors from benign lesions, especially for papillary carcinoma.

关 键 词：甲状腺肿瘤 GALECTIN-3 CK19 碘化物过氧化物酶 免疫组织化学 诊断 

分 类 号：R736.1[医药卫生—肿瘤] R977.3[医药卫生—临床医学]