胚胎细胞MAD2基因表达对染色体分离的影响  

Effects of MAD2 expression in embryonic cells on chromosome segregation

作　　者：施琼[1] 翁亚光[1] 蔡燕[1] 刘青松[1] 刘子杰[1] 

机构地区：[1]重庆医科大学临床检验诊断学实验室,重庆400016

出　　处：《第三军医大学学报》2005年第15期1544-1547,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助面上项目(30371485)~~

摘　　要：目的探讨MAD2基因表达对染色体分离的影响。方法用定量RT-PCR比较RNA干扰前后的胚胎细胞内源性基因MAD2 mRNA表达水平,同时对染色体数目异常率进行比较分析。结果干扰后MAD2基因mRNA拷贝数/GAPDH基因mRNA拷贝数的均值为干扰前的20%,染色体数目异常率均值由干扰前的4.702%上升到28.498%。经统计学分析,RNA干扰后MAD2基因mRNA水平显著下降,染色体数目异常率显著增高。结论MAD2基因表达的降低会导致染色体分离的异常,本研究为染色体分离相关蛋白功能研究建立了新的研究手段和实验评价体系。Objective To investigate the effects of MAD2 expression in embryonic cells on chromosome segregation. Methods Endogenous MAD2 mRNA level was compared by using quantitative RT-PCR and the rate of chromosome number aberration were evaluated in embryonic cells before and after RNA interference. Results The ratio of MAD2 mRNA/GAPDH mRNA after RNA interference decreased to 20% of the ratio before RNA interference （P 〈 0. 01 ）. The rate of chromosome number aberration increased from 4. 702% before RNA interference to 28. 498% after interference. Conclusion The decreased MAD2 expression might lead to abnormality of chromosome segregation.

关 键 词：MAD2基因 RNA干扰 定量RT—PCR 

分 类 号：R394.2[医药卫生—医学遗传学] Q343.2[医药卫生—基础医学]