大鼠海马CA1区神经元P2X受体对ATP、suramin的反应  

Response of P2X receptors in CA1 region of rat hippocampus to ATP and suramin

作　　者：赵延东[1] 阮怀珍[1] 

机构地区：[1]第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所,重庆400038

出　　处：《第三军医大学学报》2005年第16期1674-1677,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助面上项目(30470557)~~

摘　　要：目的研究大鼠海马CA1区神经元P2X受体对ATP的反应和suram in、iverm ectin(IVM)及低pH值对诱发电流的影响。方法采用酶加机械分离法分离后7 d的W istar大鼠海马CA1区锥体细胞,用膜片钳全细胞记录技术测定P2X受体激动剂ATP,阻断剂suram in,调节剂iverm ectin(IVM)及低pH值(pH=6.5)对跨膜电流的作用。结果分离神经元对P2X受体激动剂和阻断剂反应明显,P2X受体调节剂对不同细胞有不同的作用。结论大鼠海马CA1区锥体细胞有丰富的P2X受体表达。细胞间P2X受体的表达类型有一定差别。Objective To investigate the effects of ATP, suramin, ivermectin （ IVM ） and low pH value on pyramidal cells of rat hippocampus. Methods Pyramidal cells were rapidly dissociated from the hippocampus of 7-day postnatal rats by mechanical and enzymatic methods. Effects of ATP, suramin, IVM and low pH value on P2X receptor of neurons were studied by the technique of whole cell patch clamp. Results The transmembrane current was affected by ATP and suramin. There were different effects of IVM and low pH value on ATP induced current on different neurons. Conclusion There is extensive expression of P2X receptor on hippocampus pyramidal cells of rats. The expression of P2X receptors subunits are different among neurons.

关 键 词：P2X受体 海马 膜片钳 ATP SURAMIN 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R329.27[医药卫生—基础医学]