巢式甲基化特异性PCR检测食管癌病人血清中p16基因启动子区过甲基化  

Detection of Promoter Hypermethylation in the Serum of Esophageal Squamous Cell Carci- noma Patients by Nested Methylation-Specific-Polymerase Chain Reaction

作　　者：姚群峰[1] 康新江[1] 郝巧玲[1] 曾卫[2] 周宜开[1] 

机构地区：[1]华中科技大学同济医学院环境医学研究所,武汉430030 [2]湖北省肿瘤医院检验科

出　　处：《肿瘤防治研究》2005年第8期463-466,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(39990570)

摘　　要：目的检测食管鳞状细胞癌(squamouscellcarcinoma,SCC)患者外周血血清中p16基因启动子区的甲基化状态,探讨p16基因启动子的过甲基化在食管鳞状细胞癌筛查及早期诊断中的意义。方法利用巢式甲基化特异性PCR(nMSP)法检测食管鳞状细胞癌患者外周血血清与正常人血清中p16基因启动子的甲基化状态,并与普通甲基化特异性PCR(MSP)法进行了比较。结果56例SCC血清样品中nMSP法发现34例p16基因启动子的过甲基化,MSP法只检出15例,而22例正常人血清中都未检测到p16基因启动子的过甲基化。测序结果进一步验证了方法的可靠性。结论利用巢式MSP(nMSP)法检测外周血血清中p16基因启动子的甲基化,可为食管癌的筛查、早期诊断及预后判断提供有价值的信息。Objective This study was designed to detect the methylation status of the promoter region of p16 gene in DNA extracted serum from esophageal squamous cell carcinoma （SCC） ,and evaluate the role of p16 gene promoter hypermethylation in esophageal SCC screening and early diagnosis. Methods Nested methylation-specific PCR （nMSP） was used to detect p16 promoter hypermethylation in serum DNA from 56 esophageal squamous cell carcinoma （SCC） and control serum samples from 22 healthy individuals. Compared methylation-specific PCR（MSP）, nMSP was more sensitive. Results Aberrant promoter rmethylation of the p16 gene was found in 34 of 56 serum samples using nMSP , and only 15 cases was detected using MSP. No aberrant promoter methylation was detected in the peripheral serum of the healthy individuals. Conclusion The detection of p16 gene promoter hypermethylation in the serum of esophageal SCC patients can give the useful information for tumor early diagnosis , follow-up study of esophageal SCC patients, nMSP is a simple, sensitive ,and specific method for rapid analysis of the promoter methylation status of many genes.

关 键 词：食管鳞状细胞癌 甲基化 P16基因 聚合酶链反应 

分 类 号：R735.1[医药卫生—肿瘤]