磁性纳米颗粒介导基因治疗乳腺癌实验研究  

Magnetic Iron Oxide Nanoparticles Mediated Gene Therapy—A Study in Vitro on Breast Cancer

作　　者：韦卫中[1] 吴华[2] 李芳[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院核医学科,武汉430030 [2]福建医科大学附属厦门第一医院核医学科 [3]华中科技大学同济医学院附属同济医院妇科肿瘤中心

出　　处：《肿瘤防治研究》2005年第8期473-475,528,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30171062)

摘　　要：目的探讨新型基因载体PEI包裹的磁性纳米颗粒polyMAG-1000介导TRAIL基因治疗乳腺癌的可行性,观察TRAIL基因转染乳腺癌细胞后的治疗作用。方法以polyMAG-1000为基因载体,联结TRAIL质粒DNA后转染人乳腺癌细胞株MCF-7细胞,用Tunel法检测细胞的凋亡,用流式细胞仪检测细胞的凋亡率,以空载体作为阴性对照;脂质体转染TRAIL基因作阳性对照。结果Tunel法可见MCF-7细胞经polyMAG-1000和脂质体转染TRAIL基因后均可见发生凋亡的细胞,流式细胞仪检测polyMAG-1000转染的细胞凋亡率为25.11%±2.85%,脂质体转染的细胞凋亡率为18.31%±2.44%(P<0.05)。结论TRAIL对乳腺癌细胞MCF-7具有凋亡效应,PEI包裹的磁性纳米颗粒介导的TRAIL基因治疗在肿瘤的基因治疗中具有应用前景。Objective To study the feasibility of using TRAIL gene to treat breast cancer mediated by PEI coated magnetic iron oxide nanoparticles（polyMAG-1000）. The therapeutic efficacy was also be evaluated. Methods PEI coated magnetic iron oxide nanoparticles were used as gene carrier to transfect TRAIL gene into MCF-7 cells. The polyMAG-1000 without TRAIL gene was transfected into the tumor cells as negative control and liposome mediated TRAIL gene transfection was taken as positive control. The apoptosis of cells were detected by TUNEL method. The apoptosis ratio of tumor cells were measured with flow cytometry （FCM）. Results The apoptosis of cells was demonstrated after transfecting TRAIL gene mediated by both polyMAG-1000 and liposome. The apoptosis ratio in the group with polyMAG-1000 as gene carrier were 25. 11% ± 2.85%, whereas it was 5.06%± 1.05% in the control group with polyMAG-1000（P〈0. 0l）. The lower apoptosis ratio of 18. 31% ± 2. 44% was showed in the group with liposome as gene carrier（P〈0. 05, compared with the group with polyMAG-1000 as gene carrier）. Conclusion TRAIL gene may induce apoptosis in MCF-7 breast cancer cells. The PEI coated magnetic iron oxide nanoparticles may be a potential gene carrier with high transfection efficacy in cancer gene therapy.

关 键 词：氧化铁磁性纳米颗粒 TRAIL 乳腺癌 基因治疗 

分 类 号：R737.9[医药卫生—肿瘤]