茶树AFLP—银染技术体系与品种指纹图谱的建立  

Establishment of AFLP Technical System and Fingerprinting in Tea Plant

作　　者：黄建安[1] 黄意欢[2] 罗军武[2] 龚志华[2] 刘仲华[1] 欧秋良[1] 

机构地区：[1]教育部茶学重点实验室,湖南长沙410128 [2]湖南农业大学食品科学与技术学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2005年第4期427-430,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省自然科学基金资助项目(OOJJY20107)

摘　　要：通过对影响茶树AFLP—银染技术体系的关键因素作对比研究,建立了一套优化的茶树AFLP分子标记体系.应用该体系研究不同茶树品种的AFLP指纹,结果同一引物对不同品种扩增及不同引物对同一品种扩增都呈现出十分丰富的多态性,图谱清晰,分辨率高.The optimized AFLP analysis system was established after a comparative study of the main factors that influenced the results of AFLP silver-staining method. Fingerprints of some cultivars were obtained by this AFLP silver-staining protocol, which showed that the fingerprinting polymorphism of different cultivars from the same primer or the same cultivars from different primers was quite abundant. The AFLP patterns could be visualized unambiguously with high resolution.

关 键 词：茶树 扩增片段长度多态性 体系优化 指纹图谱 

分 类 号：S571.1[农业科学—茶叶生产加工] Q523[农业科学—作物学]