匹罗卡品致大鼠海马一氧化氮水平和caspase-3 mRNA表达的动态变化  

Dynamic changes of nitric oxide content and caspase-3 mRNA expression in hippocampus in epileptic rats induced by pilocarpine

作　　者：张映琦[1] 廖维宏[1] 曾琳[1] 伍亚民[1] 龙在云[1] 杨恒文[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第三研究室,重庆400042

出　　处：《临床神经病学杂志》2005年第4期282-284,共3页Journal of Clinical Neurology

摘　　要：目的 探讨大鼠癫痫持续状态（SE）后海马一氧化氮（NO）与caspase-3 mRNA表达的改变及相互关系。方法 采用匹罗卡品腹腔注射建立大鼠SE模型，用比色法和逆转录-聚合酶链反应（RT-PCR）技术在不同时相点检测大鼠海马NO水平与caspase-3mRNA的表达。结果 大鼠海马NO含量在SE后6h迅速升高，48～72h虽有所降低，ff}仍明显高于对照组（均P〈0．01）；SE后7d，海马NO含量仍高于正常，但差异无显著性。大鼠海马caspase-3 mRNA表达于SE后6h开始增多（P〈0．05），SE后48h达到高峰（P〈0．01），72h开始降低，SE后7d，caspase-3 mRNA表达仍高于对照组，但差异无显著性（P〉0．05）。结论 caspase-3 mRNA表达升高在NO水平升高之后，并与NO保持相似的变化趋势，提示SE后caspase-3的激活可能与NO神经毒性作用有关。Objective To probe the changes of nitric oxide （NO） content and caspase-3 mRNA expression in hippocampus and the correlation between NO and caspase-3 mRNA expression post-status epilepticus （SE）. Methods The models of SE were induced by pilocarpine. The nitric oxide content was detected by chromatometry and caspase-3 mRNA expression was observed by reverse transcription polymerase chain reaction （RT-PCR） method. Results The level of NO increased rapidly at 6 - 72 h post-SE, and was still significantly higher than that of the controls （ all P 〈0. 01 ）. It dropped to normal level at 7 d post-SE. The caspase-3 mRNA expression increased at 6 h post-SE, peaked at 48 h post-SE, then decreased gradually at 72 h post-SE. There was no statistic difference compared with control group at 7 （t post-SE （ P 〉 0. 05）. Conclusions The peak expression of nitric oxide is earlier than caspasc-3 mRNA post-SE, although they have the similar diversity tendency. Nitric oxide pathway is definitely involved in the seizure initiation and correlated with caspase-3 activation.

关 键 词：癫痫持续状态 海马 CASPASE-3 匹罗卡品 一氧化氮 

分 类 号：R742.1[医药卫生—神经病学与精神病学]