神经生长因子对大鼠脑缺血再灌注损伤的保护作用及其对Bax表达的影响  

Protective role of NGF on cerebral ischemic reperfusion injury and its effect on Bax expression

作　　者：刁士元[1] 袁颖[2] 郭海涛[1] 李峰[3] 

机构地区：[1]中国人民解放军第463医院,沈阳110042 [2]中国医科大学附属第一临床医院普外一科 [3]沈阳药科大学

出　　处：《临床神经病学杂志》2005年第4期299-301,共3页Journal of Clinical Neurology

摘　　要：目的探讨神经生长因子(NGF)对脑缺血再灌注损伤的保护作用及其对Bax表达的影响。方法采用栓线法制备大鼠局灶脑缺血再灌注模型,大鼠尾静脉注射NGF。应用免疫组化方法检测脑部神经细胞Bax表达,观察大鼠脑部病理改变。结果缺血再灌注组大鼠缺血侧半球皮质及纹状体Bax阳性细胞数为115.25±15.07,NGF组Bax阳性细胞数为70.00±12.77,两组比较差异有极显著性(P<0.01);光镜及电镜观察发现NGF组梗死区病理变化明显轻于缺血再灌注组。结论NGF对脑缺血再灌注损伤具有明确的保护作用,可减轻脑组织的损伤程度,并可减少缺血脑神经细胞Bax表达。Objective To study the protective role of nerve growth factor （NGF） on cerebral ischemic reperfusion injury and its effect on Bax expression.. Methods The models of focal cerebral ischemic reperfusion in rats were made by occlusion middle cerebral aftery using an intraluminal filament method. NGF was given through vena caudalis. The expression of Bax was studied by immunohistochemistry method and the change of brain was observed using pathological method. Results Bax positive cells in the cortex and striatum areas in ischemia- reperfusion group （ 115.25 ± 15.07 ） were more than those in NGF treatment group （ 70. 00± 12.77 ） （ P 〈 0. 01 ） , and slighter pathological change was found in NGF treatment group underwent light and electron microscope study. Conclusion NGF may play protective effect on cerebral ischemic reperfusion injury and down regulation of Bax expression.

关 键 词：神经生长因子 BAX表达 脑缺血 再灌注 

分 类 号：R743[医药卫生—神经病学与精神病学]