固定化Ralstonia metallidurans CH34降解苯酚的研究  

Study on Degrading Phenol by Immobilized Ralstonia metallidurans CH34

作　　者：吴志国[1] 王艳敏[1] 邢志华 武晓炜 宋水山[3] 高振贤[3] 马宏[3] 赵国忠 

机构地区：[1]河北工业大学化工学院生物工程系,天津300130 [2]华北制药华胜有限公司,石家庄052160 [3]河北省生物研究所环境工程室,石家庄050051

出　　处：《微生物学通报》2005年第4期31-36,共6页Microbiology China

摘　　要：将既能耐抗重金属又能降解苯酚的细菌Ralstonia m etalliduransCH34固定化以提高其降酚效率。首先通过正交实验,得到了固定化该菌种的最优制备条件,然后对固定化细胞的降酚效果进行了研究。结果表明,固定化R.m etalliduransCH34的降酚效果明显优于游离细胞;抗重金属毒性方面也有较大提高;在加入额外碳源(甲苯,柠檬酸)情况下,固定化R.m etalliduransCH34进行苯酚降解时所受影响明显要小于游离态菌。The subject of this study is to improve the ability of Ralstonia metallidurans CH34, a phenol-degrading strain with anti-heavy metal feature by immobilization. The optimal conditions for immobilizing bacterial cells were obtained with cross act experiment. The phenol-degrading efficiency of immobilized Ralstonia metallidurans CH34 was investigated. Results show that phenol-degrading efficiency of immobilized cells is obviously superior to that of the free cells ; The ability of its resistance to heavy metal have been greatly improved ; When additional carbon sources were added in the simulated waste water, the immobilized R. metallidurans CH34 were affected less than the free cells.

关 键 词：抗重金属 固定化 正交实验 额外碳源 

分 类 号：Q819[生物学—生物工程] X172[环境科学与工程—环境科学]