人参皂带Rh2联合顺铂应用对人喉癌细胞Hep-2的作用  

Effects of Ginsenoside Rh_2 Associated with Cisplatin on Human Laryngeal Squamous Cell Carcinoma Strain Hep-2

作　　者：徐洪君[1] 孟艳[2] 孙宇新[1] 

机构地区：[1]吉林大学中日联谊医院耳鼻咽喉-头颈外科,吉林长春130033 [2]吉林大学基础医学院病理生理学教研室

出　　处：《中国实验诊断学》2005年第4期584-587,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林大学青年基金资助项目(419070100056)

摘　　要：目的探讨人参皂甙Rh2与顺铂(Cisplatin,DDP)联合应用对喉癌细胞Hep-2的的作用。方法体外培养的喉癌细胞Hep-2分别加入终浓度为0.51、.0、2.0、5.0μg/ml的DDP、终浓度为10、20、30、40μg/ml的Rh2,以及终浓度为20μg/ml的Rh2分别联合终浓度为0.5、1.02、.0、5.0μg/ml的DDP,设空白对照组,待药物作用48小时后,分别用光学显微镜、MTT法、流式细胞仪(Flowcytometry,FCM)、荧光显微镜检测培养细胞的作用效果。结果光镜下可见正常喉癌细胞细胞生长密集,细胞呈梭型或多角型,细胞彼此之间相接触。在低剂量Rh2组和DDP组,可见细胞数量略减少,细胞形态无明显改变。在高剂量Rh2组和DDP组,细胞数量明显减少,可见细胞碎片。而低剂量Rh2和低剂量DDP联合应用后,可获得与高剂量Rh2组和DDP组相同的效果。MTT结果显示Rh2、DDP单独应用和联合应用都呈剂量依赖性增加抑制喉癌细胞的生长。低剂量Rh2和低剂量DDP联合应用,可明显抑制喉癌细胞生长。FCM显示Rh2和DDP单独应用,可诱导细胞凋亡,而联合应用可获得较单独应用更为明显的诱导细胞凋亡效应。吖啶橙染色显示各用药组均可见细胞胞膜出芽、染色质凝集、核片段化及凋亡小体等典型的细胞凋亡特征。结论人参皂甙Rh2及DDP均可诱导喉癌细胞凋亡,二者联合应用后,呈现出协同作用,可发挥更为明显的抗癌作用。Objective To study effects of Ginsenoside Pdh Associated with Cisplatin on human laryngeal squamous cell carcinoma strain Hep-2. Methods Using techniques of tumor cells culture in vitro, Hep-2 cells were exposed to different concentration Cisplatin as 0.5, 1.0, 2.0, 5.0μg/ml and the second groups Hep-2 culture cells were exposed to different concentration hypericin as Ginsenoside Ph2 as 10, 20, 30, 40μg/ml, the third groups Hep-2 cuhure cells were exposed to 20μg/ml Rh2 associated with different concentration Cisplatin as 0.5, 1.0, 2.0, 5.0 μg/ml. In all groups, contrast group was set up. And 48 hours later, growth characteristies of Hep-2 ceils were studied by morphological observation, fluorescence microscope, MTT assay and flow cytometry. Resuits In normal contrast group, Hep-2 cells grew intensively and contacted with each other. It could be found that cell number was declined greatly in high dose Rh2 and DDP groups while in low dose groups the modality didn＇ t changed obviously. And in the Rh2 and DDP associated groups, cell number was declined greatly and necrosis could be found. MTT assay showed that Rh2 and DDP and Rh2 associated with DDP inhibited growth of laryngeal cell with dose dependence manner. And low dose Rh2 associated with low dose DDP could inhibited growth of laryngeal cell obviously. Flow cytometry showed that Rh2 and DDP and Rh2 associated with DDP could induce apoptosis of laryngeal cell. Under fluorescence microscope, some sings of cell apoptosis including coagulation of chromatin, gragmentafion of nuclei and apoptotic body could be found. Conclusion Human laryngeal squamous cell carcinoma strain Hep-2 can be inhibited and induced into apoptosis by treated with Rhz or DDP solely and Rh2 associated with DDP in which the synergistic action could be found.

关 键 词：凋亡 人参皂甙 顺铂 喉肿瘤 

分 类 号：R739.6[医药卫生—肿瘤]