应用PCR技术诊断单纯疱疹病毒性角膜炎  

Diagnosis of herpes simplex keratitis by using PCR

作　　者：朱其新[1] 余沃莲 

机构地区：[1]肇庆市第二人民医院,广东肇庆526060

出　　处：《中国热带医学》2005年第6期1242-1242,1244,共2页China Tropical Medicine

摘　　要：目的探讨聚合酶链反应(PCR)技术诊断单纯疱疹(单疱)病毒性角膜炎的实用价值,为临床诊断提供依据和方法。方法应用PCR技术对60例按临床诊断标准诊断为单疱病毒性角膜炎患者的患眼泪液及角膜上皮进行病毒DNA检测。结果PCR检测60例患者标本结果,阳性46例占76.7%,阴性14例占23.3%。结论对单疱病毒性角膜炎诊断不能只根据其临床特征来诊断;PCR可为临床提供实验诊断依据。Objective To detect herpes simplex virus （HSV） in tear and corneal epithelium of patients with herpes simlex keratitis （HSK）. Methods Tear and corneal epithelium of 60 cases clinically diagnosed as HSK were tested by polymerase chain reaction （including 24 cases of punctate keratopathy, 29 cases of map keratopathy and 7 cases of disciform keratopathy）. Results The result was positive in 46 cases （76.7%） and negative in 14 （23.3%）, Conclusion Polymerase chain reaction is an experimental approach to diagnose HSK.

关 键 词：单疱病毒 角膜炎 泪液和角膜上皮 聚合酶链反应(PCR) 

分 类 号：R772.21[医药卫生—眼科]