反相高效液相色谱法测定牡丹皮中2种活性成分  

Determination of two main components in bark of Paeonia Suffruticosa by HPLC

作　　者：田元子[1] 王琰[1] 刘建祯[1] 杨蓓蓓[1] 石建功[1] 王慕邹[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京100050

出　　处：《中国中药杂志》2005年第16期1265-1268,共4页China Journal of Chinese Materia Medica

基　　金：国家科技项目(攻关)计划资助(99-929-01-26)

摘　　要：目的:建立HPLC测定牡丹皮中2种活性成分丹皮酚和没食子酸的含量。方法:采用AlltimaC18色谱柱(4.6mm×150mm,5μm),以水-四氢呋喃-甲醇-冰醋酸(60:20:20:0.5)为流动相,流速0.8mL·min-1,检测波长274nm。结果:丹皮酚、没食子酸的线性范围分别为0.16-2.58μg(r=0.9999,n=3),0.06-1.0μg(r=0.9999,n=3),平均回收率(n=9)分别为98.2%(RSD2.5%),98.6%(RSD3.0%)。提取方法为甲醇冷浸24h。结论:本方法准确、简捷,可为评价不同产地的牡丹皮质量提供依据。Objective： To establish a HPLC method for determination two constituents in bark of Paeonia Suffruticosa. Method： The reversed phase HPLC system consisting of an Alhima ODS column （4.6 mm×150 mm, 5μm） and a mixture of water-THF-methanol-HAc （60：20：20：0.05） as the mobile phase was used. The flow rate was 0.8 mL·min^-1 and UV detection was set at 274 nm. Result： The assay displayed good linearity over the concentration ranges of 0.06-1.0μg （r=0.9999, gallic acid） and 0.16-2.58μg （r=0.9999, paeonol） respectively. The average recoveries （n=9） of gallic acid and paeonol were 98.6% （ RSD=3.0% ）, 98.2% （ RSD=2.5% ）, respectively. The samples were extracted with methanol for 24 h bu maceration. Conclusion： The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.

关 键 词：牡丹皮 丹皮酚 没食子酸 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]