粉防己碱对获得性多药耐药小鼠S180肿瘤细胞P_(170),LRP,TOPO Ⅱ表达的调控  

The effect of tetrandrine on the expression of the P_(170),LRP and TOPO Ⅱ in S180's tumor cell induced by chemotherapy in the mice with acquired multi-drug resistance

作　　者：李贵海[1] 刘明霞[2] 孙付军[1] 王宁[3] 尹格平[4] 李晓晶[1] 李卫峰[2] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东省立医院,山东济南250021 [3]山东省卫生学校,山东济南250014 [4]济南军区总医院,山东济南250031

出　　处：《中国中药杂志》2005年第16期1280-1282,共3页China Journal of Chinese Materia Medica

基　　金：山东省自然基金资助项目(Y2002C37)

摘　　要：目的:观察粉防己碱口服给药对化疗诱导的多药耐药基因表达产物P170、肺耐药蛋白LRP和拓扑异构酶Ⅱ(TOPOⅡ)的影响。方法:以亚于治疗剂量的联合化疗PFC方案,化疗诱导建立小鼠S180肿瘤细胞多药耐药模型,同时给予粉防己碱4周,流式细胞仪荧光检测P170,LRP,TOPOⅡ。结果:粉防己碱明显降低化疗诱导后耐药细胞P170,LRP的表达率和ROPOⅡ活性。结论:粉防己碱可通过抑制耐药基因表达和DNA拓扑异构酶活性,干预化疗诱发的肿瘤细胞多药耐药的产生。Objective： To observe the effect of tetrandrine on the P170 production expressed by multi-drug resistance gene, lung resistant protein （LRP）, and topoisomeras Ⅱ and elucidate the underlying molecular mechanism. Method： Cellular model of multi-drug resistance was established in S180 tumor cell by means of the scheme of PFC chemotherapy at the dosage lower than that with curative effect. P170, LRP and TOPO Ⅱ were measured by flow cytometry after the mouse model was treated with tetrandrine for 4 weeks. Result： tetrandrine obviously reduced the enhancement of express of P170, LRP and the activity of TOPO Ⅱ in the tumor cells with multi-drug resistance induced by chemotherapy. Conclusion： Tetrandrine significandy inhibits the multi-drug resistance of tumor cells induced by chemotherapy via diminishing both the expression of multi-drug resistance gene and the activity of topoisomeras Ⅱ.

关 键 词：粉防己碱 获得性多药耐药 小鼠 P170 LRP TOPO Ⅱ 

分 类 号：R73-3[医药卫生—肿瘤]