小麦条锈菌生理小种国际鉴别寄主Spaldings Prolific中抗条锈病基因YrSpP的微卫星标记  

Microsatellite Marker of the Resistance Gene YrSpP to Wheat Stripe Rust

作　　者：关海涛[1,2] 郭玉华[3] 王悦冰[1] 刘太国[1] 蔺瑞明[1] 徐世昌[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室 [2]沈阳农业大学,沈阳110161 [3]沈阳农业大学

出　　处：《中国农业科学》2005年第8期1574-1577,共4页Scientia Agricultura Sinica

基　　金：国家"973"计划项目(G2000016200);国家"863"计划项目(2002AA245041);国家自然科学基金资助项目(30270867及30471131)

摘　　要：Spaldings Prolific是国际小麦条锈菌鉴别寄主和国内外重要抗源。以含有小麦抗条锈病基因YrSpP的近等基因系Taichung29*6/YrSpP及其轮回亲本Taichung29为材料,用目的基因所在2B染色体上88对微卫星引物对其基因组DNA进行PCR扩增和电泳分析,发现用WMC441引物在近等基因系与轮回亲本间稳定扩增出特异性DNA片段。经F2代群体162个抗、感单株检测证实,该片段位点与抗条锈病基因YrSpP连锁,遗传距离为10.9cM,确定WMC441为抗条锈病基因YrSpP的标记,并可用于该基因的检测和辅助选择。Spaldings Prolific is one of the 15 wheat cultivars in a set of differential cultivars used in Europe and the world. SSR analysis was performed between a near-isogenic line （NIL） Taichuang29＊6/Spaldings Prolific carrying the resistance gene YrSpP to wheat stripe rust and its recurrent parent Taichung29. Eighty-eight SSR primers on 2B chromosome were screened, and reproducible polymorphic DNA fragment was amplified by WMC441. Genetic linkage was tested on 162 segregating F2 plants. It showed that microsatellite marker WMC441 was linked with the resistance gene YrSpP to wheat stripe rust, and the genetic distance was 12.1 cM.

关 键 词：小麦 抗条锈病基因 YrSpP 微卫星标记 小麦条锈菌 鉴别寄主 生理小种 国际 近等基因系 微卫星引物 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]