1,2,4-三氯苯降解菌的分离及其降解质粒的研究  

Studies on isolation of 1,2,4-trichlorobenzene-degrading strain and its degradative plasmid

作　　者：宋蕾[1] 王慧[1] 施汉昌[1] 胡洪营[1] 

机构地区：[1]清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京100084

出　　处：《中国环境科学》2005年第4期385-388,共4页China Environmental Science

基　　金：国家"863"项目(2002AA601170;2002AA601150);清华大学基础研究基金(JC2003011)

摘　　要：以1,2,4-三氯苯为唯一碳源,从天津化工厂氯苯生产车间的土壤中分离到1株1,2,4-三氯苯的降解菌THSL-1.通过形态观察和16SrDNA序列测定,该菌株被初步鉴定为施氏假单胞菌(Pseudomonasstutzeri).经质粒检测,在菌株THSL-1中发现1条质粒条带,将所获得质粒转化到E.coli.JM109中,转化子能以1,2,4-三氯苯为唯一碳源生长,且对1,2,4-三氯苯有降解作用.因此,可以认为该质粒携带降解1,2,4-三氯苯的基因.选用HindIII、BamHI、XholI3种限制性内切酶分别对质粒pTHSL-1进行单酶切、双酶切,最终确定质粒平均大小为40.2kb.A 1,2,4-trichlorobenzene-degrading strain THSL-1 was isolated from the soil of Tianjin chemical plant using 1,2,4-trichlorobenzene as sole carbon source. The strain was identified preliminarily as Pseudomonas stutzeri through morphologic survey and 16S rDNA sequence determination. A plasmid strip was found from strain THSL-1 through plasmid detection. When the plasmid obtained was transformed to E. coli JMI09, the transformant could grow using 1,2,4-trichlorobenzene as sole carbon source and had degradation function of 1,2,4-trichlorobenzene. Therefore, it could be deemed that the plasmid carried the gene for 1,2,4-trichlorobenzene degradation. The plasmid average size was determined finally to be 40.2kb using selectively 3 kinds of restricted inscribed enzyme （HindⅢ,BamHI,XhoⅡ） for single cutting and double cutting the plasmid pTHSL-1 respectively.

关 键 词：1 2 4-三氯苯 16S RDNA 降解质粒 假单胞菌 

分 类 号：X144[环境科学与工程—环境科学]