红球菌CDT3降解氯氰菊酯的特性及途径  

Characters and pathway for degradation of cypermethrin by Rhodococcus sp. strain CDT3

作　　者：许育新[1] 李晓慧[1] 张明星[1] 崔中利[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院,农业部环境微生物工程重点开放实验室,江苏南京210095

出　　处：《中国环境科学》2005年第4期399-402,共4页China Environmental Science

基　　金：国家"863"项目(2004AA206071;2004AA214102;2003AA241150);江苏省科技攻关项目(BE2002345;BE2003343)

摘　　要：以一株降解氯氰菊酯的红球菌(Rhodococcussp.)CDT3为材料,研究了CDT3降解氯氰菊酯的最适条件(温度、pH值、接种量、培养时间、添加营养物).结果表明,在温度30℃,pH8.0时,CDT3对氯氰菊酯的降解率最高,添加少量葡萄糖、蛋白胨、酵母汁均能促进它对氯氰菊酯的降解.研究了CDT3降解氯氰菊酯的代谢途径,通过GC-MS分析,证实氯氰菊酯被CDT3降解后的产物是3-苯氧基苯甲酸(3-PBA)和二氯菊酸(DCVA),推测氯氰菊酯是由CDT3产生的羧酸酯酶降解的,作为验证,提取了CDT3的粗酶液,并且通过聚丙烯酰胺凝胶电泳(PAGE)检测了羧酸酯酶.Using a strain of cypermethrin-degrading Rhodococcus sp. CDT3 as material, the optimal conditions （temperature, pH value, inoculating dose, cultural time and added nutrition） for CDT3 to degrade cypermethrin was studied. In a shaky flask containing 100mg/L cypermethrin, the degrading rate of CDT3 on cypermethrin was highest at temperature 30℃, pH8.0; adding little amounts of glucose, tryptone, yeast extract could promote its degradation of cypermethrin. The metabolic pathway of cypermethrin degraded by CDT3 was studied; through GC-MS analysis, The degraded products of cypermethrin were 3-Phenoxybenzoic acid （3-PBA） and DCVA [cis,trans-3-（2,3-dichloroethenyl）-2,2-Dimethylcyclopropane-carboxylate]. It was suggested that cypermethrin came from the degrading of carboxylesterase produced from CDT3; for confirming this the crude enzyme liquor of CDT3 was extracted and carboxylesterase was detected through polyacrylanide gel electrophoresis （PAGE）.

关 键 词：红球菌 氯氰菊酯 降解特性 降解途径 羧酸酯酶 

分 类 号：X172[环境科学与工程—环境科学]